Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (10): 2132-2140.DOI: 10.1016/S2095-3119(13)60673-5

• 论文 • 上一篇    下一篇

Comparative Analysis of Gene Expression in Two Muskmelon Cultivars (Cucumis melo L.) Under Salt Stress

 WEI Shi-wei, ZHANG Fu-rong, ZHANG Yi-dong, WANG Li-min, CHEN Jia-bei , HUANG Danfeng   

  1. Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, P.R.China
  • 收稿日期:2013-09-02 出版日期:2014-10-01 发布日期:2014-10-10
  • 通讯作者: HUANG Dan-feng, Tel/Fax: +86-21-34206943, E-mail: hdf@sjtu@edu.cn
  • 作者简介:WEI Shi-wei, E-mail: weigoverment@163.com
  • 基金资助:

    This work was supported by grants from the National NaturalScience Foundation of China (31372079), the National High Technology Research and Development Program of China (863 Program, 2012AA101903), the Natural Science Foundation of Shanghai Science and Technology Committee, China (13ZR1422400), and Shanghi Graduate Education and Innovation Program (Horticulture), China.

Comparative Analysis of Gene Expression in Two Muskmelon Cultivars (Cucumis melo L.) Under Salt Stress

 WEI Shi-wei, ZHANG Fu-rong, ZHANG Yi-dong, WANG Li-min, CHEN Jia-bei , HUANG Danfeng   

  1. Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, P.R.China
  • Received:2013-09-02 Online:2014-10-01 Published:2014-10-10
  • Contact: HUANG Dan-feng, Tel/Fax: +86-21-34206943, E-mail: hdf@sjtu@edu.cn
  • About author:WEI Shi-wei, E-mail: weigoverment@163.com
  • Supported by:

    This work was supported by grants from the National NaturalScience Foundation of China (31372079), the National High Technology Research and Development Program of China (863 Program, 2012AA101903), the Natural Science Foundation of Shanghai Science and Technology Committee, China (13ZR1422400), and Shanghi Graduate Education and Innovation Program (Horticulture), China.

摘要: Salinity is one of the most important abiotic stresses that adversely affects crop growth and productivity. A subtractive suppression hybridization (SSH) library were constructed from the roots of salt-sensitive Yulu cultivar melon seedlings under salt stress; 557 high-quality expressed sequence tags (ESTs) were randomly sequenced, with an average size of 428 bp, which assembled into 68 contigs and 315 singletons. Compared with our previous SSH library generated from the salt-tolerant Bingxuecui cultivar, the proportion of transcripts involved in metabolism, protein fate, cellular communication/signal transduction mechanisms, and cell rescue/defense were 4, 1.46, 0.94, and 0.4% higher, respectively, in the salt-tolerant cultivar than the in salt-sensitive cultivar. Quantitative real-time PCR analysis of eleven transcripts revealed temporal variations in their expression in the two cultivars under salt stress. One NAC gene (JZ477011) was heterologously expressed in yeast for functional characterization, and enhanced the sensitivity of yeast cells to high-salinity to salt stress and inhibited their growth. Information regards to their functions would aid in the understanding of response mechanisms to saline stress and in the development of molecular markers for selecting salt-tolerant melon cultivars.

关键词: comparative analysis , functional characterization , gene expression , salt stress , SSH , melon cultivars

Abstract: Salinity is one of the most important abiotic stresses that adversely affects crop growth and productivity. A subtractive suppression hybridization (SSH) library were constructed from the roots of salt-sensitive Yulu cultivar melon seedlings under salt stress; 557 high-quality expressed sequence tags (ESTs) were randomly sequenced, with an average size of 428 bp, which assembled into 68 contigs and 315 singletons. Compared with our previous SSH library generated from the salt-tolerant Bingxuecui cultivar, the proportion of transcripts involved in metabolism, protein fate, cellular communication/signal transduction mechanisms, and cell rescue/defense were 4, 1.46, 0.94, and 0.4% higher, respectively, in the salt-tolerant cultivar than the in salt-sensitive cultivar. Quantitative real-time PCR analysis of eleven transcripts revealed temporal variations in their expression in the two cultivars under salt stress. One NAC gene (JZ477011) was heterologously expressed in yeast for functional characterization, and enhanced the sensitivity of yeast cells to high-salinity to salt stress and inhibited their growth. Information regards to their functions would aid in the understanding of response mechanisms to saline stress and in the development of molecular markers for selecting salt-tolerant melon cultivars.

Key words: comparative analysis , functional characterization , gene expression , salt stress , SSH , melon cultivars