Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

doi:10.1016/S2095-3119(13)60683-8

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1373-1381.DOI: 10.1016/S2095-3119(13)60683-8

Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

KE Xin, WANG Chun-yong, LI Run-dong , ZHANG Yun

1、College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, P.R.China
2、College of Land and Environment, Shenyang Agriculture University, Shenyang 110161, P.R.China

收稿日期:2013-10-11 出版日期:2014-06-01 发布日期:2014-06-06
通讯作者: ZHANG Yun, Tel: +86-24-88493104, E-mail: xingyun92757@163.com
作者简介:KE Xin, Tel: +86-24-89723608, E-mail: kex@iae.ac.cn
基金资助:
This research was supported by the Major Science and Technology Program for Water Pollution Control and Treatment, China (2012ZX07202-004-02).

Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

KE Xin, WANG Chun-yong, LI Run-dong , ZHANG Yun

1、College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, P.R.China
2、College of Land and Environment, Shenyang Agriculture University, Shenyang 110161, P.R.China

Received:2013-10-11 Online:2014-06-01 Published:2014-06-06
Contact: ZHANG Yun, Tel: +86-24-88493104, E-mail: xingyun92757@163.com
About author:KE Xin, Tel: +86-24-89723608, E-mail: kex@iae.ac.cn
Supported by:
This research was supported by the Major Science and Technology Program for Water Pollution Control and Treatment, China (2012ZX07202-004-02).

摘要/Abstract

摘要： The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37°C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L-1, respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.

关键词: oxytetracycline , anaerobic digestion stages (BDS , DMS and FDS) , PCR-DGGE , bacterial community , archaeal community

Abstract: The effects of different concentrations of oxytetracycline (OTC) on the dynamics of bacterial and archaeal communities during the mesophilic anaerobic digestion (37°C) of cow manure were investigated. Before anaerobic digestion, OTC was added to digesters at concentrations of 20, 50, and 80 mg L-1, respectively. Compared with no-antibiotic control, all methane productions underwent different levels of inhibition at different concentrations of OTC. Changes in the bacterial and archaeal communities were discussed by using PCR-denaturing gradient gel electrophoresis (DGGE). Results showed that OTC affected the richness and diversity of bacterial and archaeal communities. The bacterial genus Flavobacterium and an uncultured bacterium (JN256083.1) were detected throughout the entire process of anaerobic digestion and seemed to be the functional bacteria. Methanobrevibacter boviskoreani and an uncultured archaeon (FJ230982.1) dominated the archaeal communities during anaerobic digestion. These microorganisms may have high resistance to OTC and may play vital roles in methane production.

Key words: oxytetracycline , anaerobic digestion stages (BDS , DMS and FDS) , PCR-DGGE , bacterial community , archaeal community

KE Xin, WANG Chun-yong, LI Run-dong , ZHANG Yun. Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure[J]. Journal of Integrative Agriculture, 2014, 13(6): 1373-1381.

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Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

Effects of Oxytetracycline on Methane Production and the Microbial Communities During Anaerobic Digestion of Cow Manure

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