Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1355-1362.DOI: 10.1016/S2095-3119(13)60514-6

• 论文 • 上一篇    下一篇

Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek’s Diseases

 LI Yu-bao, LI Juan, WANG Zhi-liang , LIU Si-dang   

  1. 1、School of Agriculture, Liaocheng University, Liaocheng 252059, P.R.China
    2、School of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, P.R.China
    3、China Animal Health and Epidemiology Center, Qingdao 266032, P.R.China
  • 收稿日期:2013-02-21 出版日期:2014-06-01 发布日期:2014-06-06
  • 通讯作者: WANG Zhi-liang, Tel/Fax: +86-532-87839188, E-mail: zlwang111@163.com; LIU Si-dang, Tel/Fax: +86-538-8249526, E-mail: liusidang@126.com
  • 作者简介:LI Yu-bao, E-mail: liyubao@lcu.edu.cn; LI Juan, E-mail: li198820062011@163.com
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31101787) and the Natural Science Foundation of Shandong Province, China (ZR2010CM035).

Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek’s Diseases

 LI Yu-bao, LI Juan, WANG Zhi-liang , LIU Si-dang   

  1. 1、School of Agriculture, Liaocheng University, Liaocheng 252059, P.R.China
    2、School of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, P.R.China
    3、China Animal Health and Epidemiology Center, Qingdao 266032, P.R.China
  • Received:2013-02-21 Online:2014-06-01 Published:2014-06-06
  • Contact: WANG Zhi-liang, Tel/Fax: +86-532-87839188, E-mail: zlwang111@163.com; LIU Si-dang, Tel/Fax: +86-538-8249526, E-mail: liusidang@126.com
  • About author:LI Yu-bao, E-mail: liyubao@lcu.edu.cn; LI Juan, E-mail: li198820062011@163.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31101787) and the Natural Science Foundation of Shandong Province, China (ZR2010CM035).

摘要: To investigate the dynamic change of heat shock protein 90 (Hsp90) in the genesis and development of tumor, we successfully established tumor animal model using Marek’s disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzymelinked immunosorbent assay (ELISA) method. Our result showed that Hsp90 location in the tumor tissue was significantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most significantly increased with the development of tumor. This is the first report to show the presence of Hsp90 in tumor tissues induced by the Marek’s disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.

关键词: heat shock protein 90 , Marek&rsquo, s disease , immunohistochemistry , expression , antibody titer level

Abstract: To investigate the dynamic change of heat shock protein 90 (Hsp90) in the genesis and development of tumor, we successfully established tumor animal model using Marek’s disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzymelinked immunosorbent assay (ELISA) method. Our result showed that Hsp90 location in the tumor tissue was significantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most significantly increased with the development of tumor. This is the first report to show the presence of Hsp90 in tumor tissues induced by the Marek’s disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.

Key words: heat shock protein 90 , Marek’s disease , immunohistochemistry , expression , antibody titer level