Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (9): 1614-1619.DOI: 10.1016/s2095-3119(13)60494-3

• 论文 • 上一篇    下一篇

Characterization of MicroRNA* Species in Peking Duck Skin

 ZHANG Li, XIE Xiu-juan, JIA Shan-gang, XIAO Mei, LIN Shu-dai, AN Li-long, LUO Wen, JIA Xinzheng, NIE Qing-hua , ZHANG Xi-quan   

  1. 1.Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R.China
    2.Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China
    3.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, P.R.China
  • 收稿日期:2012-12-11 出版日期:2013-09-01 发布日期:2013-09-15
  • 通讯作者: Correspondence ZHANG Xi-quan, Tel/Fax: +86-20-85285703, E-mail: xqzhang@scau.edu.cn
  • 作者简介:ZHANG Li, E-mail: zhangli761101@163.com
  • 基金资助:

    This work was supported by the Open Fund of Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, China (201004).

Characterization of MicroRNA* Species in Peking Duck Skin

 ZHANG Li, XIE Xiu-juan, JIA Shan-gang, XIAO Mei, LIN Shu-dai, AN Li-long, LUO Wen, JIA Xinzheng, NIE Qing-hua , ZHANG Xi-quan   

  1. 1.Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R.China
    2.Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China
    3.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, P.R.China
  • Received:2012-12-11 Online:2013-09-01 Published:2013-09-15
  • Contact: Correspondence ZHANG Xi-quan, Tel/Fax: +86-20-85285703, E-mail: xqzhang@scau.edu.cn
  • About author:ZHANG Li, E-mail: zhangli761101@163.com
  • Supported by:

    This work was supported by the Open Fund of Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, China (201004).

摘要: A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3´-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.

关键词: Peking duck , miRNA star species (miRNA*) , skin , nucleotide bias

Abstract: A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3´-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.

Key words: Peking duck , miRNA star species (miRNA*) , skin , nucleotide bias