Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (10): 1721-1728.DOI: 10.1016/S1671-2927(00)8706

• 论文 • 上一篇    下一篇

Anti-Recombinant Gametocyte 56 Protein IgY Protected Chickens from Homologous Coccidian Infection

 DING Jun, LIU Qiao-rong, HAN Jin-peng, QIAN Wei-feng LIU Qun   

  1. 1.Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2011-06-13 出版日期:2012-10-01 发布日期:2012-11-12
  • 通讯作者: Correspondence LIU Qun, Tel: +86-10-62734496, E-mail: qunliu@cau.edu.cn
  • 作者简介:DING Jun, E-mail: dingjun407@163.com
  • 基金资助:

    This work was supported by the Program for Cheung Kong Scholars and Innovative Research Team in University of China (IRT0866).

Anti-Recombinant Gametocyte 56 Protein IgY Protected Chickens from Homologous Coccidian Infection

 DING Jun, LIU Qiao-rong, HAN Jin-peng, QIAN Wei-feng,  LIU Qun   

  1. 1.Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
  • Received:2011-06-13 Online:2012-10-01 Published:2012-11-12
  • Contact: Correspondence LIU Qun, Tel: +86-10-62734496, E-mail: qunliu@cau.edu.cn
  • About author:DING Jun, E-mail: dingjun407@163.com
  • Supported by:

    This work was supported by the Program for Cheung Kong Scholars and Innovative Research Team in University of China (IRT0866).

摘要: Coccidiosis is caused by intra-cellular infection of Eimeria spp., which goes through a complex life cycle in the intestinal mucosa of infected hosts. Specific immunoglobulins (IgY) could be produced in egg yolk by immunizing hens with specific antigens. In the present study, we cloned the E. maxima gam56 gene, expressed the GST-GAM56 fusion protein and raised IgY to GST-GAM56 in hens. The anti-GST-GAM56 IgY antibody was isolated and used to treat chickens infected with E. maxima oocysts. Intramuscular injection of the antibodies provided minimal protection against parasite infection. However, oral dosing of the IgY 3 or 5 d after oocyst inoculation significantly improved body weight gain, reduced oocyst output and intestinal lesion score were reduced at 3 or 5 d after oocyst challenging, compared to the untreated control group. Our findings suggest that the IgY to gam56 could be an effective prophylactic or therapeutic agent against E. maxima infection in chickens and should have a practical application value.

关键词: Eimeria maxima , recombinant GST-GAM56 , IgY , therapeutic agent

Abstract: Coccidiosis is caused by intra-cellular infection of Eimeria spp., which goes through a complex life cycle in the intestinal mucosa of infected hosts. Specific immunoglobulins (IgY) could be produced in egg yolk by immunizing hens with specific antigens. In the present study, we cloned the E. maxima gam56 gene, expressed the GST-GAM56 fusion protein and raised IgY to GST-GAM56 in hens. The anti-GST-GAM56 IgY antibody was isolated and used to treat chickens infected with E. maxima oocysts. Intramuscular injection of the antibodies provided minimal protection against parasite infection. However, oral dosing of the IgY 3 or 5 d after oocyst inoculation significantly improved body weight gain, reduced oocyst output and intestinal lesion score were reduced at 3 or 5 d after oocyst challenging, compared to the untreated control group. Our findings suggest that the IgY to gam56 could be an effective prophylactic or therapeutic agent against E. maxima infection in chickens and should have a practical application value.

Key words: Eimeria maxima , recombinant GST-GAM56 , IgY , therapeutic agent