Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

doi:10.1016/S1671-2927(11)60160-0

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (10): 1624-1629.DOI: 10.1016/S1671-2927(11)60160-0

Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

SUNDong-bo, WURui, HEXian-jing, WANGShuang, LINYun-cheng, HANXu, WANGYue-qiang, GUOTing-ting, WUGuo-jun , YANGKe-li

1.College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University

收稿日期:2010-08-24 出版日期:2011-10-01 发布日期:2011-10-18
通讯作者: Correspondence WU Rui, Professor, Tel: +86-459-6819191, Fax: +86-459-6819191, E-mail: fuhewu@126.com
作者简介:SUN Dong-bo, Ph D, E-mail: dongbosun@yahoo.com.cn
基金资助:
This work was supported by a grant from the Startup Foundation for Doctors of Heilongjiang Bayi Agricultural University, China (B2009-4).

Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

SUNDong-bo, WURui, HEXian-jing, WANGShuang, LINYun-cheng, HANXu, WANGYue-qiang, GUOTing-ting, WUGuo-jun , YANGKe-li

1.College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University

Received:2010-08-24 Online:2011-10-01 Published:2011-10-18
Contact: Correspondence WU Rui, Professor, Tel: +86-459-6819191, Fax: +86-459-6819191, E-mail: fuhewu@126.com
About author:SUN Dong-bo, Ph D, E-mail: dongbosun@yahoo.com.cn
Supported by:
This work was supported by a grant from the Startup Foundation for Doctors of Heilongjiang Bayi Agricultural University, China (B2009-4).

摘要/Abstract

摘要： Staphylococcus aureus, Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S. aureus, E. coli and B. cereus, and the detection limits were 103, 102 and 103 CFU mL-1, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had S. aureu and E. coli, 9.39% had E. coli and B. cereus, and 9.71% had S. aureus and B. cereus. The rates of infections with S. aureus, E. coli and B. cereus were 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.

关键词:

bovine endometritis, multiplex PCR, S. aureus, E. coli, B. cereus

Abstract: Staphylococcus aureus, Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows. A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S. aureus, E. coli and B. cereus, and the detection limits were 103, 102 and 103 CFU mL-1, respectively. SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases. A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR. Of the samples, 23.95% had the three kinds of bacteria detected, 17.15% had S. aureu and E. coli, 9.39% had E. coli and B. cereus, and 9.71% had S. aureus and B. cereus. The rates of infections with S. aureus, E. coli and B. cereus were 11.35, 16.18 and 9.06%, respectively. Therefore, SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows.

Key words:

bovine endometritis, multiplex PCR, S. aureus, E. coli, B. cereus

SUNDong-bo, WURui, HEXian-jing, WANGShuang, LINYun-cheng, HANXu, WANGYue-qiang, GUOTing-ting, WUGuo-jun , YANGKe-li. Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis[J]. Journal of Integrative Agriculture, 2011, 10(10): 1624-1629.

SUNDong-bo , WURui , HEXian-jing , WANGShuang , LINYun-cheng , HANXu , WANGYue-qiang , GUOTing-ting , WUGuo-jun , YANGKe-li . Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis[J]. Journal of Integrative Agriculture, 2011, 10(10): 1624-1629.

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Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus, Escherichia coli and Bacillus cereus from Cows with Endometritis

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