Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

doi:10.1016/S1671-2927(11)60155-7

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (10): 1586-1594.DOI: 10.1016/S1671-2927(11)60155-7

Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

LI Pei-pei, WANG Xiao-juan, YUAN Xu-feng, WANG Xiao-fen, CAO Yan-zuan , CUI Zong-jun

1.College of Agronomy and Biotechnology, China Agricultural University

收稿日期:2010-09-24 出版日期:2011-10-01 发布日期:2011-10-18
通讯作者: Correspondence CUI Zong-jun, Professor, Tel/Fax: +86-10-62731857, E-mail: acuizj@cau.edu.cn
作者简介:LI Pei-pei, Ph D, Tel/Fax: +86-10-62733437, E-mail: lipeipei@cau.edu.cn
基金资助:
This work was supported by the National Key Technology R&D Program of China (2008BADC4B01 and 2008BADC4B17), and the National Special Research Fund for Non-Profit Sector, China (200803033).

Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

LI Pei-pei, WANG Xiao-juan, YUAN Xu-feng, WANG Xiao-fen, CAO Yan-zuan , CUI Zong-jun

1.College of Agronomy and Biotechnology, China Agricultural University

Received:2010-09-24 Online:2011-10-01 Published:2011-10-18
Contact: Correspondence CUI Zong-jun, Professor, Tel/Fax: +86-10-62731857, E-mail: acuizj@cau.edu.cn
About author:LI Pei-pei, Ph D, Tel/Fax: +86-10-62733437, E-mail: lipeipei@cau.edu.cn
Supported by:
This work was supported by the National Key Technology R&D Program of China (2008BADC4B01 and 2008BADC4B17), and the National Special Research Fund for Non-Profit Sector, China (200803033).

摘要/Abstract

摘要： To accelerate the decomposition of wheat straw directly returned to soil, we constructed a microbial system (ADS-3) from agricultural soil containing rotting straw residues using a 40-wk limited cultivation. To assess its potential use for accelerating straw decomposing, the decomposing characteristics and the microbial composition of ADS-3 were analyzed. The results indicated that it could degrade wheat straw and filter paper by 63.8 and 80%, respectively, during 15 d of incubation. Straw hemicellulose degraded dramatically 51.2% during the first 3 d, decreasing up to 73.7% by the end of incubation. Cellulose showed sustained degradation reaching 53.3% in 15 d. Peak values of xylanase and cellulase activities appeared at 3 and 11 d, with 1.32 and 0.15 U mL-1, respectively. Estimated pH averaged 6.4-7.6 during the degradation process, which approximated acidity and alkalinity of normal soils. The microbial composition of ADS-3 was stable based on denaturing gradient gel electrophoresis (DGGE) analysis. By using bacterial 16S rRNA and fungal 26S rRNA gene clone library analysis, 20 bacterial clones and 7 fungal clones were detected. Closest identified relatives of bacteria represented by Bacillus fusiformis, Cytophaga sp., uncultured Clostridiales bacterium, Ruminobacillus xylanolyticum, Clostridium hydroxybenzoicum, and uncultured proteobacterium and the fungi were mainly identified as related to Pichia sp. and uncultured fungus.

关键词: wheat straw degradation, composite microbial system, cellulose, hemicelulose, cellulase, xylanase, microbialcomposition

Abstract: To accelerate the decomposition of wheat straw directly returned to soil, we constructed a microbial system (ADS-3) from agricultural soil containing rotting straw residues using a 40-wk limited cultivation. To assess its potential use for accelerating straw decomposing, the decomposing characteristics and the microbial composition of ADS-3 were analyzed. The results indicated that it could degrade wheat straw and filter paper by 63.8 and 80%, respectively, during 15 d of incubation. Straw hemicellulose degraded dramatically 51.2% during the first 3 d, decreasing up to 73.7% by the end of incubation. Cellulose showed sustained degradation reaching 53.3% in 15 d. Peak values of xylanase and cellulase activities appeared at 3 and 11 d, with 1.32 and 0.15 U mL-1, respectively. Estimated pH averaged 6.4-7.6 during the degradation process, which approximated acidity and alkalinity of normal soils. The microbial composition of ADS-3 was stable based on denaturing gradient gel electrophoresis (DGGE) analysis. By using bacterial 16S rRNA and fungal 26S rRNA gene clone library analysis, 20 bacterial clones and 7 fungal clones were detected. Closest identified relatives of bacteria represented by Bacillus fusiformis, Cytophaga sp., uncultured Clostridiales bacterium, Ruminobacillus xylanolyticum, Clostridium hydroxybenzoicum, and uncultured proteobacterium and the fungi were mainly identified as related to Pichia sp. and uncultured fungus.

Key words: wheat straw degradation, composite microbial system, cellulose, hemicelulose, cellulase, xylanase, microbialcomposition

LI Pei-pei, WANG Xiao-juan, YUAN Xu-feng, WANG Xiao-fen, CAO Yan-zuan , CUI Zong-jun. Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation[J]. Journal of Integrative Agriculture, 2011, 10(10): 1586-1594.

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Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

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