Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures

doi:10.1016/S1671-2927(11)60066-7

摘要/Abstract

摘要： A calcineurin B-like protein-interacting protein kinases (CIPK) gene was cloned (EU 424058) from a thermo-sensitive genic self-incompatibility (TSGI) line, HE97, in maize, which was selected for suppression subtractive hybridization (SSH) library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium (Ca²⁺). High Ca²⁺ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility (SI) under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.

关键词: CIPK gene, cloning, expression, temperature, maize

Abstract: A calcineurin B-like protein-interacting protein kinases (CIPK) gene was cloned (EU 424058) from a thermo-sensitive genic self-incompatibility (TSGI) line, HE97, in maize, which was selected for suppression subtractive hybridization (SSH) library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium (Ca²⁺). High Ca²⁺ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility (SI) under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.

Key words: CIPK gene, cloning, expression, temperature, maize

LIN Xing-e, JI Hong-qing, NIU Jun-hai, HU Yan-min, FU Zhong-jun, LIU Zong-hua, TANG Ji-hua. Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures[J]. Journal of Integrative Agriculture, 2011, 10(6): 813-819.

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