大白菜突变体lic86的激素组与转录组整合分析揭示生长素参与叶片卷曲的调控机制

doi:10.1016/j.jia.2025.12.059

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (9): 3715-3724.DOI: 10.1016/j.jia.2025.12.059

大白菜突变体lic86的激素组与转录组整合分析揭示生长素参与叶片卷曲的调控机制

收稿日期:2025-05-12 修回日期:2025-12-29 接受日期:2025-11-17 出版日期:2026-09-20 发布日期:2026-08-10

Integrated hormone and transcriptome analyses of Chinese cabbage mutant lic86 reveal the involvement of auxin in leaf curling

Xiaomeng Zhang^*, Xu Zheng^*, Shukang Bai^*, Yuanyuan Miao, Shixiong Deng, Leiguo Min, Yin Lu, Xiaocong Chang, Qiong Jia, Jianjun Zhao, Lisong Ma^#, Wei Ma^#

State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei/ Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, China

Received:2025-05-12 Revised:2025-12-29 Accepted:2025-11-17 Online:2026-09-20 Published:2026-08-10
About author:#Correspondence Wei Ma, E-mail: mawei0720@163.com; Lisong Ma, E-mail: malisong@hebau.edu.cn * These authors contributed equally to this study.
Supported by:
This work was partially funded by the National Natural Science Foundation of China (32222076, 32330096 and 32372736), the Science Research Project of Hebei Education Department (JZX2024001), the Hebei Natural Science Foundation (C2023204308), the Chunhui Talent Program of the Natural Science Foundation of Hebei (C2022204116), and the “Hundred Talents Program” for the Introduction of High-level Overseas Talents in Hebei Province (E2020100004).

摘要/Abstract

摘要：

叶片卷曲是结球类蔬菜作物的重要农艺性状，促进叶球的形成。然而，生长素调控大白菜叶片卷曲的机制尚不清晰。本研究鉴定出一份大白菜叶片内卷突变体（lic86），其叶片明显向内卷曲。与野生型（WT）相比，lic86突变体横轴方向的叶片曲率指数显著增加。激素含量检测发现，lic86叶片中只有IAA含量显著降低。在野生型和突变体中，外源喷施IAA均导致叶片外卷，而喷施生长素运输抑制剂（TIBA）则引起叶片内卷。lic86中BrAS1基因的两个拷贝表达水平显著升高，而生长素内向转运载体基因BrLAX1的表达显著降低。在野生型中通过病毒诱导的基因沉默（VIGS）技术抑制BrLAX1表达可导致叶片内卷，而在lic86突变体中沉默BrAS1基因则使叶片恢复平展表型。这些结果表明，生长素与BrAS1基因在大白菜叶片卷曲过程中发挥重要作用，为解析大白菜叶片卷曲和叶球发育的分子机制提供新见解。

Abstract:

Leaf curling is a key agronomic trait that promotes the formation of leafy heads in heading vegetable crops. However, the role of auxin in regulating leaf curling in Chinese cabbage remains largely unknown. In this study, we identified a Chinese cabbage mutant, lic86, which exhibited inward leaf curling and displayed significantly increased transverse curvature indices compared to the wild type. Additionally, among the measured hormones the concentration of indole-3-acetic acid (IAA) was significantly reduced in lic86. Exogenous application of IAA caused outward leaf curling, whereas applying the auxin transport inhibitor, TIBA, resulted in inward leaf curling in both wild type and lic86 plants. Transcriptomic analysis revealed that the differentially expressed genes between WT and lic86 were predominantly associated with hormone signal transduction pathways. Notably, the expression levels of two Brassica rapa asymmetric leaves1 (BrAS1) homologs were significantly elevated in lic86, while the expression of B. rapa LIKE-AUX1 (BrLAX1), the auxin influx carrier gene, was markedly downregulated compared to WT plants. Virus-induced gene silencing of BrLAX1 in WT plants resulted in leaf inward curvature, whereas silencing of BrAS1s in lic86 seedlings led to flattened leaf morphology. These findings offer insights into the molecular mechanism underlying leaf curling and leafy head development in Chinese cabbage.

Key words: Chinese cabbage , mutant , leaf curling , hormone , auxin

Xiaomeng Zhang, Xu Zheng, Shukang Bai, Yuanyuan Miao, Shixiong Deng, Leiguo Min, Yin Lu, Xiaocong Chang, Qiong Jia, Jianjun Zhao, Lisong Ma, Wei Ma. 大白菜突变体lic86的激素组与转录组整合分析揭示生长素参与叶片卷曲的调控机制[J]. Journal of Integrative Agriculture, 2026, 25(9): 3715-3724.

Xiaomeng Zhang, Xu Zheng, Shukang Bai, Yuanyuan Miao, Shixiong Deng, Leiguo Min, Yin Lu, Xiaocong Chang, Qiong Jia, Jianjun Zhao, Lisong Ma, Wei Ma. Integrated hormone and transcriptome analyses of Chinese cabbage mutant lic86 reveal the involvement of auxin in leaf curling[J]. Journal of Integrative Agriculture, 2026, 25(9): 3715-3724.

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大白菜突变体lic86的激素组与转录组整合分析揭示生长素参与叶片卷曲的调控机制

Integrated hormone and transcriptome analyses of Chinese cabbage mutant lic86 reveal the involvement of auxin in leaf curling

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