IIe组WRKY转录因子PlWRKY29调控芍药木质素生物合成的作用研究

doi:10.1016/j.jia.2025.09.018

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (12): 4656-4669.DOI: 10.1016/j.jia.2025.09.018

IIe组WRKY转录因子PlWRKY29调控芍药木质素生物合成的作用研究

收稿日期:2024-08-22 修回日期:2025-09-22 接受日期:2025-06-17 出版日期:2025-12-20 发布日期:2025-11-13

Regulation of lignin biosynthesis by a group IIe WRKY transcription factor PlWRKY29 in herbaceous peony

Yanqing Wu^{1, 2}, Zhanyu Dong², Yuehan Yin², Jun Tao^{1, 2}, Daqiu Zhao², Yuhan Tang^{1, 2#}

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
²College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China

Received:2024-08-22 Revised:2025-09-22 Accepted:2025-06-17 Online:2025-12-20 Published:2025-11-13
About author:Yanqing Wu, E-mail: yqwu@yzu.edu.cn; #Correspondence Yuhan Tang, E-mail: 008169@yzu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (32302586, 32330091), the China Postdoctoral Science Foundation (2023M732988, 2022M722698), the National Forest and Grass Science and Technology Innovation and Development Research Project, China (2023132012), and the Forestry Science and Technology Promotion Project of Jiangsu Province, China (LYKJ[2021]01).

摘要/Abstract

摘要：

茎秆强度是关键的农艺性状之一，对增强植株抗倒伏能力、减少弯曲变形，进而提高产量与品质具有重要作用。尽管已有研究报道多个转录因子参与植物茎秆强度的调控，但其形成的分子机制尚未完全阐明，尤其在观赏植物中研究仍较为有限。本研究在芍药中鉴定出一个IIe类WRKY转录因子PlWRKY29。在烟草中异源过表达PlWRKY29能够显著增强茎秆机械强度，并促进木质部发育、细胞壁增厚以及木质素积累；而在芍药中沉默该基因则导致相反表型。进一步机制研究表明，PlWRKY29直接结合木质素聚合基因PlLAC15的启动子区域。这些结果表明PlWRKY29正向调控植株木质素生物合成和茎秆强度，为理解植物木质素生物合成调控机制提供了新见解。

Abstract:

Stem strength represents one of the most critical agronomic traits, as it enables plants to resist lodging and bending, thereby contributing to their overall yield and quality. Various transcription factors have been shown to regulate stem strength in crops; however, the mechanisms underlying stem strength formation and regulation remain largely unexplored, particularly in ornamental plants. This study identified a group IIe WRKY transcription factor PlWRKY29 in herbaceous peony. Tobacco plants overexpressing PlWRKY29 exhibited significantly enhanced stems, expanded xylem, thickened cell walls and elevated lignin content compared to wild-type specimens. Conversely, PlWRKY29-silenced herbaceous peony demonstrated opposite characteristics. Further investigation of the regulatory mechanism revealed that PlWRKY29 bound to the promoter of PlLAC15, which encodes a monolignol polymerization gene that facilitates lignin deposition. These findings demonstrate that PlWRKY29 positively regulates lignin biosynthesis and stem strength, advancing our understanding of lignin biosynthetic regulation in plants.

Key words: herbaceous peony , stem lignification , PlWRKY29 , laccase , gene function

Yanqing Wu, Zhanyu Dong, Yuehan Yin, Jun Tao, Daqiu Zhao, Yuhan Tang. IIe组WRKY转录因子PlWRKY29调控芍药木质素生物合成的作用研究[J]. Journal of Integrative Agriculture, 2025, 24(12): 4656-4669.

Yanqing Wu, Zhanyu Dong, Yuehan Yin, Jun Tao, Daqiu Zhao, Yuhan Tang. Regulation of lignin biosynthesis by a group IIe WRKY transcription factor PlWRKY29 in herbaceous peony[J]. Journal of Integrative Agriculture, 2025, 24(12): 4656-4669.

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IIe组WRKY转录因子PlWRKY29调控芍药木质素生物合成的作用研究

Regulation of lignin biosynthesis by a group IIe WRKY transcription factor PlWRKY29 in herbaceous peony

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