Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (5): 2109-2120.DOI: 10.1016/j.jia.2025.07.017

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玉米-大豆间作并接种AMF降低镉生物有效性和提升土地生产力

  

  • 收稿日期:2025-04-01 修回日期:2025-07-14 接受日期:2025-06-13 出版日期:2026-05-20 发布日期:2026-04-11

Legume–cereal intercropping with AMF reduces cadmium bioavailability and enhances land productivity

Yanan Yang1, 2, Weizhen Chen1, 3, Zipeng Chen1, Huashou Li1#   

  1. 1 Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

    2 Kunpeng Institute of Modern Agriculture at Foshan, Foshan 528200, China

    3 Key Laboratory of Yangtze River Water Environment, Ministry of Education/College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China

  • Received:2025-04-01 Revised:2025-07-14 Accepted:2025-06-13 Online:2026-05-20 Published:2026-04-11
  • About author:Yanan Yang, E-mail: muouyingzi@163.com; #Correspondence Huashou Li, Tel: +86-20-85280211, E-mail: lihuashou@scau.edu.cn
  • Supported by:
    This work was co-funded by the Science and Technology Planning Project of Guangzhou, Guangdong Province, China (202206010176) and the National Key Research and Development Program of China (2020YFC1807805).  

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摘要：

间作体系与丛枝菌根真菌（AMF）接种联合应用，为重金属污染农田提供了一种兼具生态与经济效益的植物修复方案。然而，AMF与间作对镉土壤（Cd）生物有效性的影响尚不明确。本研究通过田间与盆栽试验，探究玉米-大豆间作与AMF接种对作物生长、Cd分配及根际土壤环境的协同效应。田间试验表明：玉米-大豆间作具有显著产量优势，土地当量比（LER）达1.62（普通玉米）与1.64（甜玉米）；间作降低大豆各部位镉积累量，其中籽粒镉积累量降低42.8%，同时维持玉米籽粒Cd浓度低于中国食品安全限值（0.20 mg kg-1，GB2762-2022）；重金属去除当量比（MRER）达1.33-1.38，证实间作在产量与Cd植物提取上的双重优势。盆栽试验表明：AMF接种联合间作体系（IN+A）使玉米增产16.4%，同时显著降低两种作物Cd积累（籽粒浓度符合安全标准）；根际分析表明IN+A处理显著改善土壤健康指标：生物有效态Cd降低34.5%，pH升高，氧化还原电位（Eh）下降，过氧化氢酶活性提升；接种处理的AMF定殖率较对照组提高2.2-4.3倍。本研究证实：经AMF强化的玉米-大豆间作体系，通过土壤碱化与氧化还原电位下降的协同调控，以及Cd向根系的定向分配，有效降低Cd生物有效性。这种微生物-植物群落协同机制，在保障作物籽粒安全的同时实现土壤Cd稳定，可为污染农田“修复-生产”同步推进提供参考。

Abstract:

The combined implementation of intercropping systems and arbuscular mycorrhizal fungi (AMF) inoculation represents a promising phytoremediation strategy for heavy metal-contaminated farmland, providing both ecological and economic benefits.  However, additional research is necessary to understand the influence of AMF and intercropping on Cd bioavailability.  This study examines the synergistic effects of maize–soybean intercropping and AMF inoculation on crop growth, cadmium (Cd) allocation patterns, and rhizosphere soil dynamics through comprehensive field and pot experiments.  Field trials revealed significant yield advantages in maize–soybean intercropping systems, with land equivalent ratios (LERs) of 1.62 (common maize) and 1.64 (sweet maize).  Intercropping decreased soybean Cd accumulation across all tissues, notably in grains (42.8% reduction), while maintaining maize grain Cd concentrations below China’s food safety threshold (0.20 mg kg–1).  The metal removal equivalent ratio (MRER) achieved 1.33–1.38 in field conditions, validating intercropping’s dual advantage in productivity and Cd phytoextraction.  Pot experiments indicated the AMF-inoculated intercropping system (IN+A) increased maize yield by 16.4% while reducing Cd accumulation in both crops, with grain concentrations meeting safety standards.  Rhizosphere analysis demonstrated IN+A treatment substantially improved soil health indicators: 34.5% reduction in bioavailable Cd, elevated pH, decreased redox potential (Eh), and enhanced catalase activity.  AMF colonization rates were 2.2–4.3 times higher in inoculated treatments (11.5–14.0%) vs. controls (3.2–5.3%).  These results establish that AMF-enhanced legume–cereal intercropping reduces Cd bioavailability through soil alkalinization (pH increase) coupled with redox potential reduction, and metal allocation plasticity redirecting Cd to root tissues.  This interaction between microbial symbiosis and plant community design stabilizes Cd in soils while maintaining crop safety (grain Cd<0.20 mg kg–1), establishing an ecoengineering approach for contaminated farmland remediation.

Key words: phytoremediation , cadmium , interplanting , maize , soybean