Homeodomain转录因子VvOCP3负调控葡萄白腐病抗性

doi:10.1016/j.jia.2025.07.001

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (9): 3451-3464.DOI: 10.1016/j.jia.2025.07.001

Homeodomain转录因子VvOCP3负调控葡萄白腐病抗性

收稿日期:2023-11-07 修回日期:2025-07-05 接受日期:2024-07-17 出版日期:2025-09-20 发布日期:2025-08-11

The homeodomain transcription factor VvOCP3 negatively regulates white rot resistance in grape

Zhen Zhang^1*, Cui Chen^1*, Changyue Jiang¹, Hong Lin¹, Yuhui Zhao¹, Yinshan Guo^{1, 2#}

¹College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
²National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design and Application Technology (Liaoning), Shenyang 110866, China

Received:2023-11-07 Revised:2025-07-05 Accepted:2024-07-17 Online:2025-09-20 Published:2025-08-11
About author:Zhen Zhang, E-mail: 1986899210@qq.com; Cui Chen, E-mail: Chencui8989293@163.com; #Correspondence Yinshan Guo, Tel: +86-24-88487143, E-mail: guoyinshan77@syau.edu.cn * These authors contributed equally to this study.
Supported by:
This work was funded by the National Natural Science Foundation of China (31972368), the China Agriculture Research System (CARS-29-yc-6), the Major Agricultural Science Projects of Liaoning Province, China (2023JH1/10200004) and the Science and Technology Program of Shenyang, China (23-410-2-03).

摘要/Abstract

摘要：

葡萄白腐病是由白腐菌Coniella diplodiella (Speg.) Sacc.（C. diplodiella）引起的一种真菌性病害，严重影响了葡萄果实的品质和产量；然而，关于植物对C. diplodiella病原菌反应的调节机制仍然知之甚少。在此，我们对葡萄（Vitis vinifera）中的一个Homeodomain（HD）转录因子VvOCP3进行了鉴定，并证明了其在C. diplodiella抗性中的重要作用。基因表达分析表明，接种C. diplodiella病原菌后，VvOCP3的表达水平显著下调。在葡萄果实瞬时注射和愈伤组织中稳定过表达的功能分析表明，VvOCP3负向调控葡萄对C. diplodiella的抗性。进一步的研究表明，VvOCP3直接与VvPR1（病原相关蛋白 1）的启动子结合并抑制其转录，从而降低了对C. diplodiella的抗性。此外，VvOCP3还能与2C型蛋白磷酸酶VvABI1相互作用，后者是ABA信号通路的负调节因子。综上所述，我们的研究结果表明VvOCP3在葡萄抗白腐病中起着至关重要的调节作用，为通过基因工程调节VvOCP3的表达水平来培育具有更强白腐病抗性的葡萄品种提供了理论指导。

Abstract:

Grape white rot is a fungal disease caused by Coniella diplodiella (Speg.) Sacc. that seriously affects fruit quality and yield; however, the underlying mechanism governing the plant response to C. diplodiella pathogens is still poorly understood. Here, we characterized a homeodomain (HD) transcription factor from grape (Vitis vinifera), VvOCP3, and demonstrated its significance in C. diplodiella resistance. Expression analysis showed that VvOCP3 expression was significantly down-regulated upon inoculation with C. diplodiella. Functional analysis with transient injection in grape berries and stable overexpression in grape calli demonstrated that VvOCP3 negatively regulates grape resistance to C. diplodiella. Further studies showed that VvOCP3 directly binds to the promoter of VvPR1 (pathogenesis-related protein 1) and inhibits its expression, resulting in reduced resistance to C. diplodiella. In addition, VvOCP3 can interact with the type 2C protein phosphatase VvABI1, which is a negative modulator of the ABA signaling pathway. In summary, our findings suggest that VvOCP3 plays a crucial role in regulating white rot resistance in grape, and offer theoretical guidance for developing grape cultivars with enhanced C. diplodiella resistance by regulating the expression of VvOCP3.

Key words: grape , white rot , VvOCP3 , VvPR1 , VvABI1

Zhen Zhang, Cui Chen, Changyue Jiang, Hong Lin, Yuhui Zhao, Yinshan Guo. Homeodomain转录因子VvOCP3负调控葡萄白腐病抗性[J]. Journal of Integrative Agriculture, 2025, 24(9): 3451-3464.

Zhen Zhang, Cui Chen, Changyue Jiang, Hong Lin, Yuhui Zhao, Yinshan Guo. The homeodomain transcription factor VvOCP3 negatively regulates white rot resistance in grape[J]. Journal of Integrative Agriculture, 2025, 24(9): 3451-3464.

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Homeodomain转录因子VvOCP3负调控葡萄白腐病抗性

The homeodomain transcription factor VvOCP3 negatively regulates white rot resistance in grape

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