两个与春小麦赤霉病抗性相关的QTL位点的定位与验证

doi:10.1016/j.jia.2024.12.021

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (8): 3126-3138.DOI: 10.1016/j.jia.2024.12.021

两个与春小麦赤霉病抗性相关的QTL位点的定位与验证

收稿日期:2024-08-05 修回日期:2024-12-19 接受日期:2024-10-22 出版日期:2026-08-20 发布日期:2026-07-08

Identification and validation of two QTLs associated with Fusarium head blight resistance in spring wheat (Triticum aestivum L.)

Cong Li^{2, 3*}, Lei Wu^1*, Xinyao He², Yi He¹, Peng Jiang¹, Jian Ma³, Pawan K. Singh^2#, Xu Zhang^{1, 4#}

¹CIMMYT-JAAS Joint Center for Wheat Diseases, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
²International Maize and Wheat Improvement Center, Texcoco C.P. 56237, Mexico
³State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China
⁴Collaborative Innovation Center for Modern Crop Production Co-sponsored by Jiangsu Province and Ministry of Agriculture and Rural Affairs, Nanjing 210095, China

Received:2024-08-05 Revised:2024-12-19 Accepted:2024-10-22 Online:2026-08-20 Published:2026-07-08
About author:Cong Li, E-mail: licong20210317@163.com; #Correspondence Pawan K. Singh, E-mail: pk.singh@cgiar.org; Xu Zhang, Tel: +86-25-84390293, Fax: +86-25-84390321, E-mail: xuzhang@jaas.ac.cn
Supported by:
This research has been funded by the National Key R&D Program of China (2022YFD1400105), the International Scientific and Technological Cooperation Projects of Jiangsu Province, China (BZ2024043), the Seed Industry Revitalization Project of Jiangsu Province, China (JBGS2021006), and the Jiangsu Agricultural Science and Technology Innovation Fund, China (CX (22)2005).

摘要/Abstract

摘要：

赤霉病（FHB）是全球小麦生产中极具破坏性的重要病害，严重威胁粮食安全与保障。本研究利用南京8611/Ocoroni 重组自交系（RIL）群体的269个F6株系进行赤霉病抗性QTL位点鉴定。所有株系连续3年分别在南京以单花滴注法、在墨西哥以孢子喷雾法进行田间赤霉病抗性鉴定，同时利用小麦55K 单核苷酸多态性（SNP）芯片构建高密度遗传图谱。共检测到13个QTL，分别位于1B, 2D, 3B, 5D, 6D和 7A染色体，其中两个主效QTL位点qfhb.CIM-2D.1 和qdon.CIM-3B.1得到稳定表达。条件QTL分析表明，位点qfhb.CIM-2D.1通过降低FHB严重度进而减少DON含量，而qdon.CIM-3B.1则通过直接控制DON积累提高赤霉病抗性。聚合qfhb.CIM-2D.1 和qdon.CIM-3B.1位点可以显著提高小麦对赤霉病和DON的抗性。此外，成功开发了与qfhb.CIM-2D.1 和qdon.CIM-3B.1分别紧密连锁的竞争性等位基因特异性PCR（KASP）标记KASP-1369 和 KASP-8394，并在相关的遗传群体中得到有效验证。本研究结果拓宽了赤霉病抗性研究遗传基础，开发的分子标记将有效应用于小麦标记辅助育种。

Abstract:

Fusarium head blight (FHB) is one of the most important and destructive wheat diseases worldwide, threatening both food security and safety. In this study, a recombinant inbred line (RIL) population with 269 F₆ lines developed from a cross between ‘Nanjing 8611’ and ‘Ocoroni’ was used to map quantitative trait loci (QTLs) for FHB resistance. Field FHB trials were conducted for three years in Nanjing, China, using point inoculation, and two years in Mexico with spray inoculation. A high-density genetic map was constructed for the RIL population using the wheat 55 K single nucleotide polymorphism (SNP) array. A total of 13 QTLs were detected on chromosomes 1B, 2D, 3B, 5D, 6D, and 7A, among which two major QTLs, QFhb.CIM-2D.1 and QDon.CIM-3B.1, were stably expressed in this study. Conditional QTL analysis suggested that QFhb.CIM-2D.1 contributes to reduced deoxynivalenol (DON) content via decreasing FHB severity, whereas QDon.CIM-3B.1 contributed to FHB resistance by directly controlling DON accumulation. Stacking of QFhb.CIM-2D.1 and QDon.CIM-3B.1 exhibited a marked increase in resistance against both FHB and DON. Furthermore, two Kompetitive Allele-Specific PCR (KASP) markers, KASP-1369 and KASP-8394, tightly linked to QFhb.CIM-2D.1 and QDon.CIM-3B.1, respectively, were developed and successfully validated in their respective genetic populations. Altogether, these results broaden the understanding of the genetic basis of resistance to FHB, and the developed markers are valuable for marker-assisted wheat breeding.

Key words: wheat , Fusarium head blight , QTL mapping , deoxynivalenol (DON) , disease resistance

Cong Li, Lei Wu, Xinyao He, Yi He, Peng Jiang, Jian Ma, Pawan K. Singh, Xu Zhang. 两个与春小麦赤霉病抗性相关的QTL位点的定位与验证[J]. Journal of Integrative Agriculture, 2026, 25(8): 3126-3138.

Cong Li, Lei Wu, Xinyao He, Yi He, Peng Jiang, Jian Ma, Pawan K. Singh, Xu Zhang. Identification and validation of two QTLs associated with Fusarium head blight resistance in spring wheat (Triticum aestivum L.)[J]. Journal of Integrative Agriculture, 2026, 25(8): 3126-3138.

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两个与春小麦赤霉病抗性相关的QTL位点的定位与验证

Identification and validation of two QTLs associated with Fusarium head blight resistance in spring wheat (Triticum aestivum L.)

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