玉米/大豆间作下作物根系互作与根际细菌群落受不同大豆品种调控

doi:10.1016/j.jia.2024.12.018

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (10): 4048-4062.DOI: 10.1016/j.jia.2024.12.018

玉米/大豆间作下作物根系互作与根际细菌群落受不同大豆品种调控

收稿日期:2024-10-14 修回日期:2024-12-16 接受日期:2024-11-06 出版日期:2025-10-20 发布日期:2025-09-24

Soybean variety influences the advantages of nutrient uptake and yield in soybean/maize intercropping via regulating root-root interaction and rhizobacterial composition

Tianqi Wang^{1, 2, 3*}, Jihui Tian^1*, Xing Lu^{1, 2, 3*}, Chang Liu^{1, 2, 3}, Junhua Ao⁴, Huafu Mai^{1, 2, 3}, Jinglin Tan^{1, 2, 3}, Bingbing Zhang^{1, 2, 3}, Cuiyue Liang^{1, 2, 3#}, Jiang Tian^{1, 2, 3#}

¹Root Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
²State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China
³Guangdong Engineering Technology Research Center of Low Carbon Agricultural Green Inputs, South China Agricultural University, Guangzhou 510642, China
⁴Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, China

Received:2024-10-14 Revised:2024-12-16 Accepted:2024-11-06 Online:2025-10-20 Published:2025-09-24
About author:#Correspondence Cuiyue Liang, E-mail: liangcy@scau.edu.cn; Jiang Tian, E-mail: jtian@scau.edu.cn * These authors contributed equally to this study.
Supported by:
This work was supported by the National Key Research and Development Program of China (2021YFF1000504 and 2023YFD1901300), the National Natural Science Foundation of China (32172658, 32172659 and 32302662), the Natural Science Foundation of Guangdong Province, China (2021A1515010826).

摘要/Abstract

摘要：

玉米/大豆间作体系在发展中国家得到普遍应用，但鲜有研究解析玉米与不同大豆品种间作时根-根互作所介导的养分效率与根际微生物组成上的差异。本研究通过田间试验比较了两个大豆（Glycine max）品种BD2和YC03-3，以及一个“华珍”品种鲜食玉米（Zea mays）在单、间作栽培模式下的生长与产量变化。与单作相比，BD2/玉米间作下两种作物的植株生物量和氮含量均显著提高，但在YC03-3/玉米间作中则无明显影响。与单作相比，与玉米间作后BD2产量提高了37.5%。此外，玉米与BD2间作后根长增加了19.2%-29.1%，间作BD2的根体积提高了19.0%-39.4%，而在YC03-3/玉米间作中根系性状无显著变化。同时，玉米与BD2间作时表现出根系回避现象，而与YC03 - 3间作时则表现为空间竞争。16S rRNA测序结果表明，与单作体系相比，BD2/玉米间作中的根际细菌群落组成比YC03-3/玉米间作中的变化更为显著。在BD2/玉米间作中，大部分BD2根际富集的优势细菌与其生物量及磷、氮含量呈正相关。而与BD2间作的玉米在根际招募了根瘤菌目（Rhizobiales）和变形菌门（Proteobacteria）细菌，它们分别与玉米生物量与氮含量及土壤速效氮呈正相关。总之，不同大豆品种通过根-根互作和改变根际细菌群落组成调控了玉米/大豆间作的资源利用优势。本文强调了根系性状与根际细菌群落之间的联系，体现了酸性土壤中通过选择合适的作物品种来优化间作体系的重要性。

Abstract:

Maize/soybean intercropping systems are commonly used in developing countries, but few studies have been performed to elucidate the differences in nutrient efficiency and rhizosphere microbiome, especially when maize is intercropped with different soybean varieties. In this study, field experiments were conducted to compare the growth and yield of two soybean (Glycine max) varieties, BD2 and YC03-3, and one maize (Zea mays) variety, Huazhen, in mono-cropped and intercropped cultures. The plant biomass and N content of both crops in BD2/maize intercropping were significantly improved compared to their monoculture, but no such effects were observed in the plants of YC03-3/maize intercropping. The yield of BD2 intercropped with maize exhibited a 37.5% increment above that of BD2 in monoculture. Moreover, 19.2–29.1% longer root length of maize and 19.0–39.4% larger root volume of BD2 were observed in BD2/maize intercropping than in monoculture, but no growth advantage was observed in YC03-3/maize intercropping. Maize showed root avoidance when intercropped with BD2, but space competition when intercropped with YC03-3. 16S rRNA amplicon sequencing showed that compared with the monoculture system, rhizobacteria community composition in BD2/maize intercropping changed more significantly than that of the YC03-3/maize intercropping system. In BD2/maize intercropping, most of the rhizobacteria community biomarker bacteria of BD2 were positively correlated with plant biomass, as well as plant P and N content. Maize tended to recruit Rhizobiales and Proteobacteria, which showed positive correlation with plant biomass and N content, respectively, as well as soil available N. In conclusion, soybean varieties determined the advantages of maize/soybean intercropping through root–root interactions and modification of rhizobacteria communities. Our insight emphasizes a linkage between root traits and the rhizobacteria community, which shows the importance of optimizing intercropping systems by selection of appropriate crop varieties.

Key words: maize/soybean intercropping , roots , bacterial community , soybean variety , maize

Tianqi Wang, Jihui Tian, Xing Lu, Chang Liu, Junhua Ao, Huafu Mai, Jinglin Tan, Bingbing Zhang, Cuiyue Liang, Jiang Tian. 玉米/大豆间作下作物根系互作与根际细菌群落受不同大豆品种调控[J]. Journal of Integrative Agriculture, 2025, 24(10): 4048-4062.

Tianqi Wang, Jihui Tian, Xing Lu, Chang Liu, Junhua Ao, Huafu Mai, Jinglin Tan, Bingbing Zhang, Cuiyue Liang, Jiang Tian. Soybean variety influences the advantages of nutrient uptake and yield in soybean/maize intercropping via regulating root-root interaction and rhizobacterial composition[J]. Journal of Integrative Agriculture, 2025, 24(10): 4048-4062.

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玉米/大豆间作下作物根系互作与根际细菌群落受不同大豆品种调控

Soybean variety influences the advantages of nutrient uptake and yield in soybean/maize intercropping via regulating root-root interaction and rhizobacterial composition

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