新疆蔬菜根结线虫发生及遗传多样性分析

doi:10.1016/j.jia.2024.12.008

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (6): 2475-2484.DOI: 10.1016/j.jia.2024.12.008

新疆蔬菜根结线虫发生及遗传多样性分析

收稿日期:2024-04-15 修回日期:2024-12-10 接受日期:2024-10-15 出版日期:2026-06-20 发布日期:2026-05-07

The occurrence and genetic diversity of vegetable root-knot nematodes in Xinjiang Uygur Autonomous Region

Junhui Zhou^1*, Yuxuan Zhao^{2, 3*}, Wenfang Luo^1*, Hudie Shao⁴, WeiHe¹, Deliang Peng², Wenkun Huang², Huiqin Wang⁶, Honghai Zhao³, Jianjun Xu^1#, Huan Peng^{1, 2#}

¹Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis of the Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Korla of the Ministry of Agriculture and Rural Affairs, Urumqi 830091, China
²State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
³Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China
⁴College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China
⁵Agricultural Technology Extension Station of Xinjiang Uygur Autonomous Region, Urumqi 830000, China
⁶Xinjiang Plant Protection Station, Urumqi 830000, China

Received:2024-04-15 Revised:2024-12-10 Accepted:2024-10-15 Online:2026-06-20 Published:2026-05-07
About author:Junhui Zhou, E-mail: junhuiqzhou@163.com; Yuxuan Zhao, E-mail: zhaoyuxuan0323@163.com; Wenfang Luo, E-mail: lf576263465@163.com; #Correspondence Huan Peng, Tel: +86-10-62815576, E-mail: penghuan@caas.cn; Jianjun Xu, E-mail: Xjj72@163.com * These authors contributed equally to this study.
Supported by:
This work was supported by the Open Fund of the Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture, China (KFJJ202102), the Major Science and Technology Projects in Xinjiang Uygur Autonomous Region, China (2022A02005-3), the Tianchi Talent Introduction Program in Xinjiang Uyghur Autonomous Region, China, the “Group Team” Aid Xinjiang Project of Xinjiang Uygur Autonomous Region and the Science and the Technology Innovation Project of the Chinese Academy of Agricultural Sciences, China.

摘要/Abstract

摘要：

根结线虫（RKNs）是造成全球经济损失最为严重的植物寄生性线虫。新疆占中国陆地面积的六分之一，目前缺乏新疆蔬菜根结线虫发生、分布和遗传多样性的综合数据。因此，明确新疆根结线虫种类及其遗传多样性对制定全面的防控策略至关重要。在2021年至2023年期间，我们对新疆14个地州86个县收集了130个样本进行了研究，旨在全面了解新疆蔬菜根结线虫的发生、分布、危害及其种类。通过形态学和分子生物学手段对新疆根结线虫的种类及其遗传多样性进行了研究。结果表明，在新疆哈密、吐鲁番、伊犁、巴州、和田、阿克苏、喀什和克州等地采集到的130个样本中检测到有57个样本被根结线虫侵染，研究表明新疆蔬菜产区的根结线虫病害正在扩展。新疆蔬菜根结线虫的种类为南方根结线虫（Meloidogyne incognita）和北方根结线虫(M. hapla)，其中南方根结线虫为优势种群。对南方根结线虫的COI区域进行系统发育分析，结果表明，中国新疆与中国东南部RKN种群之间存在显著进化和遗传差异。基于COI基因单倍型分析表明，南方根结线虫种群可划分为三大主要谱系：亚洲、欧美和非洲。我们研究发现南方根结线虫种群间有显著的基因流发生，其中从欧洲和美国向亚洲（尤其是从中国东南部向新疆）进行了漂移。我们研究结果表明，根结线虫对新疆蔬菜危害在持续加剧，实施有效的防控措施对缓解根结线虫的扩散至关重要。

Abstract:

Root-knot nematodes (RKNs) are the most economically damaging plant-parasitic nematodes globally. Xinjiang, encompassing one-sixth of China’s landmass, currently lacks comprehensive data regarding the occurrence, distribution, and genetic variation of RKNs infecting vegetables within its borders. Hence, identifying RKNs species and genetic diversity is crucial for devising comprehensive management strategies. Between 2021 and 2023, we present a survey of 130 samples, collected from 86 counties across 14 cities in Xinjiang, China, aiming to comprehensively understand the occurrence, distribution, damage, and species of vegetable RKNs. The results indicated that 57 out of 130 samples collected from the regions of Hami, Tulufan, Ili, Bayingol, Hotan, Aksu, Kashgar, and Kizilsu in Xinjiang were infected by RKNs, suggesting an expansion of RKN disease in the vegetable-producing regions of Xinjiang. The infected vegetable roots were found to harbor Meloidogyne incognita and M. hapla, with M. incognita being the most prevalent species. A phylogenetic analysis of the COI region revealed significant evolutionary divergence between M. incognita populations from Xinjiang and those from southeastern provinces. Haplotype analysis of the COI gene revealed that M. incognita populations are categorized into three major lineages: Asia, Europe, and a combined lineage encompassing both America and Africa. Notable gene flow patterns were observed among M. incognita populations, with significant migrations from Europe and America to Asia, specifically from the southeastern China towards Xinjiang. This study’s findings indicate a consistent increase in the detrimental effects of vegetables production caused by RKNs in Xinjiang. Implementing effective prevention and control measures is crucial to mitigate the spread of RKNs.

Key words: root-knot nematode , vegetables , occurrence , genetic diversity , Xinjiang

Junhui Zhou, Yuxuan Zhao, Wenfang Luo, Hudie Shao, Wei He, Deliang Peng, Wenkun Huang, Huiqin Wang, Honghai Zhao, Jianjun Xu, Huan Peng. 新疆蔬菜根结线虫发生及遗传多样性分析[J]. Journal of Integrative Agriculture, 2026, 25(6): 2475-2484.

Junhui Zhou, Yuxuan Zhao, Wenfang Luo, Hudie Shao, Wei He, Deliang Peng, Wenkun Huang, Huiqin Wang, Honghai Zhao, Jianjun Xu, Huan Peng. The occurrence and genetic diversity of vegetable root-knot nematodes in Xinjiang Uygur Autonomous Region[J]. Journal of Integrative Agriculture, 2026, 25(6): 2475-2484.

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新疆蔬菜根结线虫发生及遗传多样性分析

The occurrence and genetic diversity of vegetable root-knot nematodes in Xinjiang Uygur Autonomous Region

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