马铃薯自交不亲和位点的遗传多样性分析

doi:10.1016/j.jia.2024.12.011

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (4): 1448-1460.DOI: 10.1016/j.jia.2024.12.011

马铃薯自交不亲和位点的遗传多样性分析

收稿日期:2023-12-07 接受日期:2024-03-20 出版日期:2025-04-20 发布日期:2025-03-17

Genetic diversity of the self-incompatibility locus in diploid potato

Xinyu Jing^{1, 2}, Duoduo Qian^{2, 3}, Xiuhan Jiang², Pei Wang², Huihui Bao⁴, Dianjue Li⁴, Yanhui Zhu², Guangtao Zhu⁴, Chunzhi Zhang²^#

¹ National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
² Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
³ State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475004, China
⁴ Yunnan Key Laboratory of Potato Biology, The AGISCAAS–YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming 650000, China

Received:2023-12-07 Accepted:2024-03-20 Online:2025-04-20 Published:2025-03-17
About author:Xinyu Jing, E-mail: jingxinyu@webmail.hzau.edu.cn; #Correspondence Chunzhi Zhang, E-mail: zhangchunzhi01@caas.cn
Supported by:
This study was supported by the National Natural Science Foundation of China (32372695 and 32488302), the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ZDRW202404).

摘要/Abstract

摘要：

配子体型自交不亲和（Gametophytic self-incompatible，GSI）是茄科植物普遍存在的一种自交不亲和性，它由一个核糖核酸酶（S-RNase）和多个F-box（SLF）组成的S位点控制；然而，二倍体马铃薯的S位点遗传多样性尚不清楚。本研究对194份二倍体马铃薯花柱转录组进行无参拼接，结果共鉴定到21种S-RNase等位基因型，其中7个S-RNase等位基因是首次被鉴定，分别是S15、S16、S17、S18、S19、S20和S21。等位基因频率结果显示，S2等位基因型在整个群体中基因频率最高，达到11.89%。随后，分析纯合S2自交系的基因组拼接数据显示S2位点包含12个SLF基因。对4个纯合S位点共线性分析发现8个SLF相对保守。Ka和Ks计算结果显示不同S-RNase和同种单倍型内一簇SLF受到同种进化轨迹，但不同单倍型的同类SLF受到不同的进化轨迹。以上研究不仅对二倍体马铃薯种质S-RNase等位基因型进行了深入研究，而且对自交不亲和S位点组成和进化进行了分析，为二倍体马铃薯杂交育种提供了理论基础。

Abstract:

Re-domestication of diploid potato (Solanum tuberosum) into a seed crop is an innovative breeding method to accelerate genetic improvement. Seed propagation would allow hybrid production and mix superior alleles. However, almost all diploid potatoes in nature are self-incompatible (SI). Gametophytic self-incompatible (GSI) is a widespread SI in Solanaceae and is controlled by the S locus that contains a ribonuclease (S-RNase) and multiple F-box (SLFs); however, the genetic diversity of the S locus in potato is unclear. This study identified 21 S-RNase alleles involved in SI from 194 diploid potato accessions by large-scale transcriptome sequencing. The levels of amino acid similarity among different S-RNase proteins varied from 31.3 to 95.8%. S2 allele is the most widespread in 194 diploid potatoes and is mainly distributed in the S. tuberosum Group Phureja. Based on genomic annotation and expression analysis, we identified 12 potential functional SI male-determinant genes, S-locus F-box (SLFs), encoding F-box proteins in the S2 locus on a genomic region of approximately 13 Mb. Comparative genomics analysis showed that eight SLF genes are relatively conserved among four homozygous S locus. The Ka and Ks analysis suggested that S-RNase and intra-haplotypic SLF genes have co-evolved. These findings help select suitable pollinators, combine more hybrid combinations, and fully use heterosis to accelerate diploid potato breeding.

Key words: potato , self-incompatibility , S-RNase , SLF

Xinyu Jing, Duoduo Qian, Xiuhan Jiang, Pei Wang, Huihui Bao, Dianjue Li, Yanhui Zhu, Guangtao Zhu, Chunzhi Zhang. 马铃薯自交不亲和位点的遗传多样性分析[J]. Journal of Integrative Agriculture, 2025, 24(4): 1448-1460.

Xinyu Jing, Duoduo Qian, Xiuhan Jiang, Pei Wang, Huihui Bao, Dianjue Li, Yanhui Zhu, Guangtao Zhu, Chunzhi Zhang. Genetic diversity of the self-incompatibility locus in diploid potato[J]. Journal of Integrative Agriculture, 2025, 24(4): 1448-1460.

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马铃薯自交不亲和位点的遗传多样性分析

Genetic diversity of the self-incompatibility locus in diploid potato

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