小麦-冰草2P易位系广谱免疫叶锈病基因的区段定位

doi:10.1016/j.jia.2024.12.001

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (5): 2015-2018.DOI: 10.1016/j.jia.2024.12.001

小麦-冰草2P易位系广谱免疫叶锈病基因的区段定位

收稿日期:2024-07-15 修回日期:2024-12-04 接受日期:2024-10-21 出版日期:2025-05-20 发布日期:2025-04-18

Identification of a genomic region controlling broad-spectrum immunity to leaf rust in wheat–Agropyron cristatum 2P translocation lines

Xiajie Ji^{1, 3*}, Shirui Xu^1*, Zongyao Wang¹, Haiming Han¹, Jinpeng Zhang¹, Shenghui Zhou¹, Baojin Guo¹, Xinming Yang¹, Xiuquan Li¹, Xiaomin Guo¹, Taiguo Liu^2#, Lihui Li^1#, Weihua Liu^1#

¹State Key Laboratory of Crop Gene Resources and Breeding/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
²State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
³Institute of Agricultural Information and Economics, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Received:2024-07-15 Revised:2024-12-04 Accepted:2024-10-21 Online:2025-05-20 Published:2025-04-18
About author:Xiajie Ji, E-mail: jixiajie003@126.com; Shirui Xu, E-mail: xushirui1105@163.com; #Correspondence Weihua Liu, Tel: +86-10-62176077, Fax: +86-10-62189650, E-mail: liuweihua@caas.cn; Lihui Li, Tel: +86-10-62186670, E-mail: lilihui@caas.cn; Taiguo Liu, Tel: +86-10-62815618, E-mail: tgliu@ippcaas.cn * These authors contributed equally to this study.
Supported by:
This research was funded by the National Natural Science Foundation of China (32272083).

摘要/Abstract

摘要：

叶锈病是危害小麦生产的主要病害之一，栽培小麦广谱高抗叶锈病基因匮乏。小麦-冰草易位系2PT-5具有来自冰草2P长臂对小麦叶锈病广谱免疫的区段。为了准确定位该抗叶锈病基因区段，本研究利用辐照诱导获得的小麦-冰草2P易位系TT-5、TT-3和TT-26分离群体进行叶锈菌接种鉴定，结合基因组原位杂交（GISH）、分子标记检测和基因组重测序对抗叶锈病基因进行物理定位。将抗叶锈病定位区间由原来的82 Mb缩小至9.2 Mb，定位于2P长臂物理位置926.4~935.6 Mb区间。目标区间内注释了64个冰草特异基因，包含6个典型抗病基因，其中有2个编码NLR蛋白的基因和2个编码受体激酶的基因响应叶锈菌的侵染。抗叶锈病基因目标区段的定位，为进一步克隆和解析转移到小麦中的这一广谱抗叶锈病基因奠定了重要的基础。

Xiajie Ji, Shirui Xu, Zongyao Wang, Haiming Han, Jinpeng Zhang, Shenghui Zhou, Baojin Guo, Xinming Yang, Xiuquan Li, Xiaomin Guo, Taiguo Liu, Lihui Li, Weihua Liu. 小麦-冰草2P易位系广谱免疫叶锈病基因的区段定位[J]. Journal of Integrative Agriculture, 2025, 24(5): 2015-2018.

Xiajie Ji, Shirui Xu, Zongyao Wang, Haiming Han, Jinpeng Zhang, Shenghui Zhou, Baojin Guo, Xinming Yang, Xiuquan Li, Xiaomin Guo, Taiguo Liu, Lihui Li, Weihua Liu. Identification of a genomic region controlling broad-spectrum immunity to leaf rust in wheat–Agropyron cristatum 2P translocation lines[J]. Journal of Integrative Agriculture, 2025, 24(5): 2015-2018.

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小麦-冰草2P易位系广谱免疫叶锈病基因的区段定位

Identification of a genomic region controlling broad-spectrum immunity to leaf rust in wheat–Agropyron cristatum 2P translocation lines

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