非典型蛋白激酶基因GhABC1K2-A05和GhABC1K12-A07的敲低使棉花对盐和PEG胁迫更敏感

doi:10.1016/j.jia.2024.01.035

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (10): 3370-3386.DOI: 10.1016/j.jia.2024.01.035

非典型蛋白激酶基因GhABC1K2-A05和GhABC1K12-A07的敲低使棉花对盐和PEG胁迫更敏感

收稿日期:2023-07-25 接受日期:2023-11-13 出版日期:2024-10-20 发布日期:2024-09-04

Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress

Caixiang Wang^1*, Meili Li^1*, Dingguo Zhang³, Xueli Zhang¹, Juanjuan Liu¹, Junji Su^{1, 2#}

¹ State Key Laboratory of Aridland Crop Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
² Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China
³ College of Biology and Geographical Sciences, Yili Normal University, Yining 835000, China

Received:2023-07-25 Accepted:2023-11-13 Online:2024-10-20 Published:2024-09-04
About author:Caixiang Wang, E-mail: wangcaix@gsau.edu.cn; #Correspondence Junji Su, Tel/Fax: +86-931-7631875, E-mail: sujj@gsau.edu.cn * These authors contributed equally to this study.
Supported by:
The work was supported by the State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, China (GSCS-2019-10), the National Natural Science Foundation of China (31801414 and 32260478), the Gansu Province Science and Technology Program, China (20JR10RA531), the Natural Science Foundation of Xinjiang Uygur Autonomous Region, China (2022D01E103), and the Education Technology Innovation Project of Gansu Province, China (2022QB-076).

摘要/Abstract

摘要：

bc₁复合体激酶（ABC1K）是一种非典型蛋白激酶(aPK)，在植物线粒体或质体胁迫反应中起着至关重要的作用，但ABC1Ks在棉花（Gossypium spp.）中对胁迫的反应知之甚少。我们在陆地棉（Gossypium hirsutum L.）中验证了40个ABC1K基因，发现GhABC1K基因不均匀地分布在17条染色体上。GhABC1K家族成员包含35对同源基因，并通过片段复制进行扩增，其启动子序列包含多种与激素或应激反应相关的顺式调控元件。qRT-PCR结果显示，暴露于不同的胁迫下，大多数GhABC1K基因上调表达。至少三种胁迫处理上调了GhABC1K2-A05和GhABC1K12-A07的表达。通过病毒诱导的基因沉默（VIGS）进一步对这些基因进行了功能分析。与对照相比，在NaCl和PEG胁迫下，GhABC1K2-A05-和GhABC1K12-A07沉默棉花植株的丙二醛（MDA）含量升高，过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性降低，叶绿素和可溶性糖含量降低。此外，胁迫后应激标记基因（GhDREB2A、GhSOS1、GhCIPK6、GhSOS2、GhWRKY33和GhRD29A）在GhABC1K2-A05-和GhABC1K12-A07沉默植株中的表达显著下调。结果表明，GhABC1K2-A05和GhABC1K12-A07基因的敲低使棉花对盐和PEG胁迫更加敏感。这些发现可以为深入研究GhABC1K基因在棉花对非生物胁迫的响应和抗性中的作用提供有价值的信息。

Abstract:

Activity of bc₁ complex kinase (ABC1K) is an atypical protein kinase (aPK) that plays a crucial role in plant mitochondrial and plastid stress responses, but little is known about the responses of ABC1Ks to stress in cotton (Gossypium spp.). Here, we identified 40 ABC1Ks in upland cotton (Gossypium hirsutum L.) and found that the GhABC1Ks were unevenly distributed across 17 chromosomes. The GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental duplication. The GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress responses. The qRT-PCR results revealed that most GhABC1Ks were upregulated by exposure to different stresses. GhABC1K2-A05 and GhABC1K12-A07 expression levels were upregulated by at least three stress treatments. These genes were further functionally characterized by virus-induced gene silencing (VIGS). Compared with the controls, the GhABC1K2-A05- and GhABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde (MDA) contents, lower catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG stress. In addition, the expression levels of six stress marker genes (GhDREB2A, GhSOS1, GhCIPK6, GhSOS2, GhWRKY33, and GhRD29A) were significantly downregulated after stress in the GhABC1K2-A05- and GhABC1K12-A07-silenced lines. The results indicate that knockdown of GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress. These findings can provide valuable information for intensive studies of GhABC1Ks in the responses and resistance of cotton to abiotic stresses.

Key words: cotton , ABC1K , abiotic stress responses , expression patterns , virus-induced gene silencing (VIGS)

Caixiang Wang, Meili Li, Dingguo Zhang, Xueli Zhang, Juanjuan Liu, Junji Su. 非典型蛋白激酶基因GhABC1K2-A05和GhABC1K12-A07的敲低使棉花对盐和PEG胁迫更敏感[J]. Journal of Integrative Agriculture, 2024, 23(10): 3370-3386.

Caixiang Wang, Meili Li, Dingguo Zhang, Xueli Zhang, Juanjuan Liu, Junji Su. Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress[J]. Journal of Integrative Agriculture, 2024, 23(10): 3370-3386.

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非典型蛋白激酶基因GhABC1K2-A05和GhABC1K12-A07的敲低使棉花对盐和PEG胁迫更敏感

Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress

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