基于TOR信号通路对中黑盲蝽防控的RNAi靶标筛选

doi:10.1016/j.jia.2024.08.005

摘要/Abstract

摘要：

中黑盲蝽Adelphocoris suturalis是一种重要的杂食性农业害虫。由于转Bt抗虫棉花商业化种植以及Bt棉田化学杀虫剂使用的减少，中黑盲蝽作为次要害虫发生日益加重。因此，研究新的可持续防控中黑盲蝽的有效方法迫在眉睫。本研究通过注射雷帕霉素靶蛋白（TOR）信号通路抑制剂，发现TOR信号通路在中黑盲蝽的生殖调控中起着关键作用。基因沉默（RNAi）在病虫害防控中具有较大的应用潜力。本研究进一步通过基因克隆、RNAi、实时荧光定量PCR（qPCR）等技术发现，分别沉默TOR信号通路基因TOR、脑Ras同源蛋白（Rheb）和S6蛋白激酶（S6K）能够显著降低该虫的繁殖力。此外，将TOR基因的双链RNA（dsRNA）通过纳米递送系统处理中黑盲蝽成功抑制了该虫的卵巢发育。这些结果为了解中黑盲蝽的生殖调控奠定了理论基础，并为了高效防控中黑盲蝽提供了新的思路与RNAi靶标。

Abstract:

Adelphocoris suturalis is a polyphagous pest that is increasingly causing severe economic damage due to more frequent outbreaks. The development of non-target resistance to commercial Bacillus thuringiensis (Bt) cotton has further exacerbated its pest status and amplified the need for more sustainable methods of control. RNA interference (RNAi)-based pest management strategies, such as root soaking and transgenic plants that express dsRNAs, have proven to be reliable, eco-friendly pest control strategies. To identify new RNAi targets for potential A. suturalis population control, we investigated the target of rapamycin (TOR) signaling pathway. A critical role for this pathway in A. suturalis reproductive regulation was suggested by pharmacological analyses. Subsequent RNAi-mediated knockdown of the A. suturalis TOR pathway genes TOR, Ras homolog enriched in the brain (Rheb), and ribosomal S6 kinase (S6K) reduced fertility. Moreover, a spray-induced and nanocarrier-delivered gene silencing (SI-NDGS) system targeting TOR successfully suppressed ovarian development, which demonstrates its effectiveness as a pest control target. These results provide a critical foundation for understanding reproductive regulation in A. suturalis and introduce new candidates for RNAi-based A. suturalis management.

Key words: Adelphocoris suturalis , target of rapamycin (TOR) signaling , reproductive regulation , RNAi targets , nanocarrier-mediated RNAi

Wanying Zheng, Panyang Jiao, Xiaona Xu, Weihua Ma, J. Joe Hull, Hongxia Hua, Lizhen Chen. 基于TOR信号通路对中黑盲蝽防控的RNAi靶标筛选[J]. Journal of Integrative Agriculture, 2025, 24(4): 1529-1541.

Wanying Zheng, Panyang Jiao, Xiaona Xu, Weihua Ma, J. Joe Hull, Hongxia Hua, Lizhen Chen. Identification of a TOR signaling pathway gene as a candidate target for reproductive management of Adelphocoris suturalis[J]. Journal of Integrative Agriculture, 2025, 24(4): 1529-1541.

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