基于生理性状挖掘普通菜豆抗旱相关QTL

doi:10.1016/j.jia.2024.03.079

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (11): 3657-3671.DOI: 10.1016/j.jia.2024.03.079

基于生理性状挖掘普通菜豆抗旱相关QTL

收稿日期:2023-12-28 接受日期:2024-03-07 出版日期:2024-11-20 发布日期:2024-10-10

Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean

Lei Wu^{1, 2}, Yujie Chang², Lanfen Wang², Shumin Wang², Jing Wu^{2, 3}^#

¹Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
²Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
³SXAU–ICS (Shanxi Agricultural University–Institute of Crop Sciences) Joint Research Center of Minor Crops, Shanxi Agricultural University, Taiyuan 030031, China

Received:2023-12-28 Accepted:2024-03-07 Online:2024-11-20 Published:2024-10-10
About author:#Correspondence Jing Wu, Tel: +86-10-62175628, E-mail: wujing@caas.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2019YFD1001300 and 2019YFD1001305), the Fundamental Research Funds for the Central Universities, China (SWU-KQ22042), the China Agriculture Research System of MOF and MARA (CARS-08), and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.

摘要/Abstract

摘要：

抗旱性遗传改良是普通菜豆育种的主要目标之一，因此，亟需挖掘与抗旱性相关的分子标记，通过分子辅助育种技术促进普通菜豆抗旱育种。本研究测定了210份普通菜豆的脯氨酸、海藻糖、棉子糖和水苏糖含量，发现不同处理条件下的脯氨酸、海藻糖、棉子糖和水苏糖含量变化较大。对照条件下脯氨酸含量的变异系数最小（21.21%），水苏糖含量的变异系数最大（78.69%），干旱条件下海藻糖含量的变异系数最大（50.08%），脯氨酸含量的变异系数最小（20.11%）。通过全基因组关联分析鉴定出32个数量性状位点与抗旱性相关，其中7个与已知抗旱相关遗传位点重叠。在染色体Pv01、Pv07和Pv11上发现了4个热点区域。结合基因功能注释、同源基因功能研究以及基因表达谱数据，在候选区段中鉴定出多个候选基因，包括编码MYB、bZIP、bHLH、ERF和蛋白激酶的基因。其中Phvul.001G189400、Phvul.007G273000和Phvul.008G270500分别被注释为bZIP、ERF和WRKY，这些基因参与拟南芥的干旱胁迫反应，在普通菜豆中受干旱胁迫诱导。基于候选基因区域的显著SNP，可以将供试材料分成不同的单倍型，表型分析结果表明不同单倍型之间存在显著差异。这些结果丰富了普通菜豆抗旱性遗传信息，鉴定出来的候选基因和优良的自然变异将有助于普通菜豆抗旱性改良。

Abstract:

Genetic improvement of drought resistance is one of the main breeding goals for common bean, so molecular markers must be identified to facilitate drought resistance breeding. In this study, we evaluated the proline, trehalose, raffinose, and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four. The coefficients of variation ranged from 21.21% for proline content to 78.69% for stachyose content under well-watered conditions, and from 20.11% for proline content to 50.08% for trehalose content under drought stress. According to our genome-wide association analysis, 32 quantitative trait loci were associated with drought resistance, seven of which overlapped with known loci. Four hotspot regions were identified at Pv01, Pv07 and Pv11. A set of candidate genes was identified, including genes encoding MYB, bZIP, bHLH, ERF, and protein kinases. Among these genes, Phvul.001G189400, Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP, ERF and WRKY, respectively. These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean. Significant SNPs in six candidate gene regions formed different haplotypes, and phenotypic analysis revealed significant differences among the haplotypes. These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.

Key words: common bean , drought resistance , GWAS , physiological trait

Lei Wu, Yujie Chang, Lanfen Wang, Shumin Wang, Jing Wu. 基于生理性状挖掘普通菜豆抗旱相关QTL[J]. Journal of Integrative Agriculture, 2024, 23(11): 3657-3671.

Lei Wu, Yujie Chang, Lanfen Wang, Shumin Wang, Jing Wu. Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean[J]. Journal of Integrative Agriculture, 2024, 23(11): 3657-3671.

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基于生理性状挖掘普通菜豆抗旱相关QTL

Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean

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