脂动激素（AKH）信号通路调控飞蝗成虫抗旱性

doi:10.1016/j.jia.2024.03.019

摘要/Abstract

摘要：

全球气候变化导致干旱事件愈发严峻，从而严重威胁各种生物的生存；然而，关于生物抗旱性分子调控机制的研究较少。本研究对不同干旱（食物介导）环境下飞蝗成虫的生物学性状进行系统比较，发现脂动激素（AKH）信号通路对飞蝗成虫抗旱性具有重要调控作用。主要结果如下，干旱导致飞蝗成虫的失水率降低、体重损失率增加和寿命缩短。转录组分析显示，干旱选择系和对照系飞蝗存在组织特异性基因表达变异。特别重要的是，AKH基因表达受到干旱选择和干旱胁迫的显著诱导。进一步结合RNA干扰和海藻糖外源补充，发现AKH1、AKH2或脂动激素受体（AKHR）基因干扰显著加速干旱环境下飞蝗成虫的水分散失和死亡，外源补充海藻糖能够改善由AKH或AKHR干扰引起的不利影响。该研究结果表明AKH/AKHR信号通路介导的海藻糖代谢对飞蝗抵抗干旱胁迫至关重要，促进了对生物抗旱性调控机制的理解。

Abstract:

Drought events have become more severe under climate change, and this can pose a major threat to the survival of various organisms. The molecular mechanisms involved in dehydration resistance are not well known. Here, adults of the migratory locust, Locusta migratoria, were subjected to food-mediated dehydration, and adipokinetic hormone (AKH) signaling was found to play a key role in regulating dehydration resistance. Specifically, dehydration shortened the lifespan, increased the body weight loss, and reduced the water loss rate in adult locusts. Global transcriptome profiles revealed variations in tissue-specific gene expression between dehydration-resistant locusts and normal locusts. Importantly, dehydration selection and exposure induced prominent expression of AKH genes in the retrocerebral complex of adult locusts. Furthermore, individual knockdown of AKH1, AKH2, or AKH receptor (AKHR) accelerated water loss and shortened the lifespan of adult locusts under dehydration conditions, and trehalose supplementation ameliorated the negative effects caused by interference with AKH or AKHR. These findings demonstrated that AKH/AKHR signaling-dependent trehalose metabolism plays a crucial role in regulating locust dehydration resistance and thus provide novel insights into the regulatory mechanism underlying drought resistance.

Key words: adipokinetic hormone , adipokinetic hormone receptor , trehalose , dehydration resistance , Locusta migratoria

. 脂动激素（AKH）信号通路调控飞蝗成虫抗旱性[J]. Journal of Integrative Agriculture, 2024, 23(9): 3104-3117.

Xianliang Huang, Dai Shi, Kai Deng, Shuzhen Jia, Ding Ding, Li Hou, Bing Chen. Adipokinetic hormone signaling regulates adult dehydration resistance in the migratory locust[J]. Journal of Integrative Agriculture, 2024, 23(9): 3104-3117.

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