C型凝集素CTL-S15基因表达降低甜菜夜蛾对苏云金芽孢杆菌的敏感性

doi:10.1016/j.jia.2024.01.024

摘要/Abstract

摘要：

C型凝集素（CTL）是昆虫先天免疫反应的模式识别受体，可能与Cry毒素竞争碱性磷酸酶受体，从而降低其对昆虫的毒力。然而，到目前为止，CTL是否会影响甜菜夜蛾对Bt的敏感性尚不清楚。本研究首先鉴定了甜菜夜蛾33个CTL家族基因。根据糖识别域（CRD）的结构和数量将其分为三类:（1）含有单个CRD的S型19个；（2）含有两个串联排列的CRD的 IML型8个；（3）含有CRD和其他结构域的X型6个。 RT-qPCR分析显示，Bt和Cry1Ab毒素处理幼虫后，SeCTL-S15、IML-4和CTL-X6的基因表达水平明显上调。甜菜夜蛾不同组织和发育阶段的基因表达分析表明，只有SeCTL-S15主要在中肠和幼虫期表达。RNA干扰SeCTL-S15基因表达，幼虫对Bt的敏感性显著增加，存活率和体重均显著降低。这些结果表明，CTL-S15可能在甜菜夜蛾幼虫对Bt的低敏感性中起重要作用。同时该研究结果为探索昆虫CTL的功能提供了新的见解。

关键词: Spodoptera exigua , Bacillus thuringiensis , susceptibility , C-type lectins

Abstract:

It has been reported that C-type lectins (CTLs), which are pattern recognition receptors of the insect innate immunity response, may compete with Cry toxin for the receptor alkaline phosphatase to decrease its toxicity in insects. However, to date, which CTLs affect larval susceptibility to Bt in Spodoptera exigua is not clear. In this study, 33 CTL genes were identified from S. exigua. Based on the number of carbohydrate-recognition domains (CRDs) and the domain architectures, they were classified into three groups: (1) nineteen CTL-S (single-CRD), (2) eight immulectin (dual-CRD) and (3) six CTL-X (CRD with other domains). RT-qPCR analysis revealed that expression levels of SeCTL-S15, IML-4 and CTL-X6 were upregulated after challenge with Bt and Cry1Ab. Tissue and developmental stage expression analysis showed that only SeCTL-S15 was mainly expressed in the midgut and larva, respectively. Knockdown of SeCTL-S15 significantly increased Bt susceptibility, as indicated by reduced survival and larval weight. These results suggest that CTL-S15 might play a vital role in the low susceptibility of larvae to Bt in S. exigua. Our results provide new insights into CTL function in insects.

Key words: Spodoptera exigua , Bacillus thuringiensis , susceptibility , C-type lectins

Jianqiang Bao, Yuxuan Chen, Suwan Jiang, Rui Liu, Xi Zhang, Fangzheng Zhang, Zhiwei Chen, Chen Luo, Hailong Kong. C型凝集素CTL-S15基因表达降低甜菜夜蛾对苏云金芽孢杆菌的敏感性[J]. Journal of Integrative Agriculture, 2024, 23(9): 3078-3088.

Jianqiang Bao, Yuxuan Chen, Suwan Jiang, Rui Liu, Xi Zhang, Fangzheng Zhang, Zhiwei Chen, Chen Luo, Hailong Kong. Induced CTL-S15 gene expression by Bacillus thuringiensis declines susceptibility in Spodoptera exigua[J]. Journal of Integrative Agriculture, 2024, 23(9): 3078-3088.

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