综合转录组学和代谢组学鉴定调控刺梨槲皮素衍生物合成的关键基因

doi:10.1016/j.jia.2023.11.022

摘要/Abstract

摘要：

刺梨果实富含黄酮类化合物，但对其生物合成途径知之甚少。在本研究中，我们采用转录组学和代谢组学研究了刺梨果实发育五个阶段黄酮类化合物的动态变化。黄酮类化合物及其生物合成相关基因在不同发育阶段的丰度发生显著变化，并鉴定出大量槲皮素衍生物。WGCNA结果中发现三个与刺梨中黄酮类化合物的丰度显著相关的基因表达模块，并在这些模块中鉴定了三个直接参与槲皮素衍生物合成的结构UDP-糖基转移酶基因。此外，我们发现RrBEH4、RrLBD1和RrIF8可以显著促进槲皮素衍生物生物合成下游基因的表达。总之，这些结果为刺梨中黄酮类化合物的代谢和槲皮素衍生物的积累提供了新的见解。

Abstract:

Rosa roxburghii fruit is rich in flavonoids, but little is known about their biosynthetic pathways. In this study, we employed transcriptomics and metabolomics to study changes related to the flavonoids at five different stages of R. roxburghii fruit development. Flavonoids and the genes related to their biosynthesis were found to undergo significant changes in abundance across different developmental stages, and numerous quercetin derivatives were identified. We found three gene expression modules that were significantly associated with the abundances of the different flavonoids in R. roxburghii and identified three structural UDP-glycosyltransferase genes directly involved in the synthesis of quercetin derivatives within these modules. In addition, we found that RrBEH4, RrLBD1 and RrPIF8 could significantly increase the expression of downstream quercetin derivative biosynthesis genes. Taken together, these results provide new insights into the metabolism of flavonoids and the accumulation of quercetin derivatives in R. roxburghii.

Key words: Rosa roxburghii , Quercetin derivatives , Weighted gene co-expression network analysis , Transcription factor , Transcriptome , Metabolome

. 综合转录组学和代谢组学鉴定调控刺梨槲皮素衍生物合成的关键基因[J]. Journal of Integrative Agriculture, 2024, 23(3): 876-887.

Liyao Su, Min Wu, Tian Zhang, Yan Zhong, Zongming (Max) Cheng.

Identification of key genes regulating the synthesis of quercetin derivatives in Rosa roxburghii through integrated transcriptomics and metabolomics [J]. Journal of Integrative Agriculture, 2024, 23(3): 876-887.

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