定位葡萄无核性状的KASP标记开发

doi:10.1016/j.jia.2023.10.014

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (11): 3269-3283.DOI: 10.1016/j.jia.2023.10.014

定位葡萄无核性状的KASP标记开发

收稿日期:2023-05-26 接受日期:2023-10-03 出版日期:2023-11-20 发布日期:2023-11-08

Development and application of KASP marker for high throughput detection of the seedless trait in grapevine

WANG Fu-qiang¹, BIAN Lu¹, QIU Peng-peng¹, GUO Shuo¹, GUO Jing-han¹, GUO Chen-shuo¹, JIANG Jian-fu², LIU Chong-huai², WANG Yong³, LIU Guo-tian¹, WANG Yue-jin¹, XU Yan^1#

¹ Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs/State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
²Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, P.R.China
³Research Institute of Grape and Melon of Xinjiang Uygur Autonomous Region, Shanshan 838200, P.R.China

Received:2023-05-26 Accepted:2023-10-03 Online:2023-11-20 Published:2023-11-08
About author:WANG Fu-qiang, E-mail: wangfuqiang@nwafu.edu.cn; #Correspondence XU Yan, E-mail: yan.xu@nwafu.edu.cn
Supported by:
This work was supported by the earmarked fund for the China Agriculture Research System of MOF and MARA (CARS-29-yc-3 ), Project of Agricultural Breeding in Ningxia Hui Autonomous Region (NXNYYZ20210104) and the Key Industrial Innovation Chain Project in Shaanxi Province (2021ZDLNY04-08).

摘要/Abstract

摘要：

分子标记辅助选择(MAS)可以显著提高无核葡萄的育种效率，加快育种进程。本研究基于VviAGL11基因的单碱基突变位点（Chr.18:26889437（A/C））开发了KASP_VviAGL11和VviAGL11_410标记，并以SSR标记p3_VvAGL11、5U_VviAGL11标记作对比，分别在101份葡萄品种和81份葡萄F₁杂交株系进行检测。结果认为KASP_VviAGL11和VviAGL11_410均检测出A等位基因时与葡萄无核性状紧密关联，且准确率为100%，而p3_VvAGL11、5U_VviAGL11由于容易产生假阳性导致准确率偏低。然后通过评估不同标记使用的技术优缺点，明确了KASP_VviAGL11标记具有更加简单、经济、高效、精确的优势。最终，本研究优化了以KASP_VviAGL11标记为核心的无核葡萄分子标记辅助选择育种过程，为加快无核葡萄新品种培育进程提供关键技术支持。

Abstract:

Molecular marker-assisted selection (MAS) can significantly accelerate and improve the efficiency of the breeding process in seedless grape cultivars. In this study, we developed the KASP_VviAGL11 and VviAGL11_410 markers based on a single nucleotide polymorphism (SNP) site (Chr18: 26889437 (A/C)) of the VviAGL11 gene, and compared them with previously reported SSR markers p3_VvAGL11 and 5U_VviAGL11 by testing 101 cultivars and 81 F₁ hybrid progenies. The results showed that both of the proposed markers obtained 100% accuracy rates in detecting allele A, which was closely associated with the seedless trait in grapes, while p3_VvAGL11 and 5U_VviAGL11 had lower accuracy rates due to their tendency to produce false positives. After careful evaluation of the technical advantages and disadvantages associated with these markers, we concluded that KASP_VviAGL11 was superior in terms of simplicity, cost-effectiveness, efficiency, and accuracy. Thus, we optimized the process of molecular MAS for seedless grapes, focusing on the KASP_VviAGL11 marker as a central component, to provide key technical support for the development of new seedless grape cultivars.

Key words: seedless grape , MAS , KASP , SSR

. 定位葡萄无核性状的KASP标记开发[J]. Journal of Integrative Agriculture, 2023, 22(11): 3269-3283.

WANG Fu-qiang, BIAN Lu, QIU Peng-peng, GUO Shuo, GUO Jing-han, GUO Chen-shuo, JIANG Jian-fu, LIU Chong-huai, WANG Yong, LIU Guo-tian, WANG Yue-jin, XU Yan. Development and application of KASP marker for high throughput detection of the seedless trait in grapevine[J]. Journal of Integrative Agriculture, 2023, 22(11): 3269-3283.

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定位葡萄无核性状的KASP标记开发

Development and application of KASP marker for high throughput detection of the seedless trait in grapevine

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