一种新的禾谷镰孢菌分泌蛋白FgHrip1诱导植物免疫反应

doi:10.1016/j.jia.2023.08.009

摘要/Abstract

摘要：

禾谷镰孢菌是小麦赤霉病的主要致病菌，它在侵染植物的过程中，会分泌大量的化合物来影响植物的抗病性。本研究从禾谷镰孢菌的发酵液中分离纯化出一种能够诱导烟草细胞坏死的蛋白FgHrip1。通过蛋白质质谱技术获得了FgHrip1的肽段序列，并根据NCBI的数据库比对，获得了FgHrip1基因序列。对FgHrip1基因序列进行克隆，并用原核表达系统获得了重组表达蛋白FgHrip1-His-Trx。进一步的生物测定分析发现，重组蛋白FgHrip1诱导烟草的早期免疫反应，包括活性氧的产生、胼胝质积累、电解质外流和相关抗性基因的上调表达。此外，FgHrip1增强烟草对丁香假单胞菌和烟草花叶病毒的抗性；FgHrip1处理小麦穗后诱导小麦产生免疫反应，激活了水杨酸通路，提高了小麦对赤霉病的抗性。虽然在禾谷镰孢菌侵染小麦时，FgHrip1基因被显著诱导上调表达，但FgHrip1敲除突变株的致病性明显减弱，表明FgHrip1同时作为毒力因子参与禾谷镰孢菌的致病性。研究结果表明FgHrip1诱导植物免疫，增强植物的广谱抗病性，为赤霉病的生物防治策略提供了新的见解。

Abstract:

Fusarium graminearum, the primary pathogenic fungus responsible for Fusarium head blight (FHB) in wheat, secretes abundant chemical compounds that interact with host plants. In this study, a secreted protein FgHrip1, isolated from the culture filtrate of F. graminearum, was found to induce typical cell death in tobacco. The FgHrip1 gene was then cloned and expressed in Escherichia coli. Further bioassay analysis showed that the recombinant FgHrip1 induced early defense induction events, such as reactive oxygen species (ROS) production, callose deposition, and up-regulation of defense-related genes in tobacco. Furthermore, FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae pv. tabaci 6605 (Pst. 6605) and tobacco mosaic virus (TMV). FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation and developed immunity against FHB infection. Whereas the expression of FgHrip1 was induced during the infection process, the deletion of the gene impaired the virulence of F. graminearum. Our results suggest that FgHrip1 triggers immunity and induces disease resistance in tobacco and wheat, thereby providing new insight into strategy for biocontrol of FHB.

Key words: FgHrip1 , secreted protein , plant immunity , Fusarium graminearum , wheat

Zhenchao Fu, Huiqian Zhuang, Vincent Ninkuu, Jianpei Yan, Guangyue Li, Xiufen Yang, Hongmei Zeng. 一种新的禾谷镰孢菌分泌蛋白FgHrip1诱导植物免疫反应[J]. Journal of Integrative Agriculture, 2024, 23(11): 3774-3787.

Zhenchao Fu, Huiqian Zhuang, Vincent Ninkuu, Jianpei Yan, Guangyue Li, Xiufen Yang, Hongmei Zeng. A novel secreted protein FgHrip1 from Fusarium graminearum triggers immune responses in plants[J]. Journal of Integrative Agriculture, 2024, 23(11): 3774-3787.

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