VGLL2基因参与调控固始鸡骨骼肌生长发育

doi:10.1016/j.jia.2023.06.017

摘要/Abstract

摘要：

先前的研究表明，VGLL2是VGLL家族的一员，在动物骨骼肌的生长发育中发挥着重要作用，但其在鸡骨骼肌发育中的具体作用尚不清楚。本研究的主要目的是探讨VGLL2在鸡骨骼肌发育和鸡原代成肌细胞增殖分化中的生物学功能。在本研究中，我们通过CCK8、EdU和流式细胞术分析检测了VGLL2在过表达和干扰VGLL2后对成肌细胞增殖的影响，采用间接免疫荧光法IF检测VGLL2对成肌细胞分化的影响。qRT‒PCR和苏木精-伊红（H&E）染色用于评估VGLL2过表达对鸡骨骼肌生长速率和骨骼肌纤维特性的影响。结果表明，VGLL2抑制了鸡原代成肌细胞的增殖，但促进了这些细胞的分化。有趣的是，鸡骨骼肌纤维发育因VGLL2的过表达而显著增强。本研究首次从体内和体外对鸡VGLL2基因在骨骼肌中的作用进行了探究，并且数据表明VGLL2基因可能是改善家禽肌肉质量的有用标志物。本研究结果为家禽肌肉发育的研究提供了新的思路，对家禽生产具有一定的指导意义。同时，也为进一步完善我国地方鸡分子育种计划、建立我国地方鸡肌肉发育调控网络提供了理论依据。

Abstract:

Previous studies have shown that VGLL2, a member of the mammalian Vestigial-like (VGLL) family, plays important roles in the growth and development of animal skeletal muscle, but its specific role in the development of chicken skeletal muscle is unclear. The main goal of this study was to explore the biological functions of VGLL2 in the development of chicken skeletal muscle and the proliferation and differentiation of skeletal muscle cells in vitro. In this study, we detected the effect of VGLL2 on the proliferation of myoblasts by CCK8, EdU and flow cytometry analyses after overexpressing and interfering with VGLL2. Indirect immunofluorescence was used to detect the effect of VGLL2 on the differentiation of myoblasts. qRT-PCR and hematoxylin and eosin (H&E) staining were used to evaluate the effects of VGLL2 overexpression on the growth rate and muscle fiber structure of chicken skeletal muscle. The results showed that VGLL2 inhibited the proliferation of primary cultured chicken myoblasts and promoted the differentiation of these cells. Interestingly, food intake and muscle fiber development were significantly enhanced by the overexpression of VGLL2 in chickens. Taken together, these data demonstrate that the VGLL2 gene may be a useful marker for improving muscle mass in poultry.

Key words: chicken , VGLL2 , myoblast , proliferation , differentiation

Wenya Li, Haoxiang Ma, Yanxing Wang, Yushi Zhang, Yang Liu, Ruili Han, Hong Li, Hanfang Cai, Xiaojun Liu, Xiangtao Kang, Ruirui Jiang, Zhuanjian Li. VGLL2基因参与调控固始鸡骨骼肌生长发育[J]. Journal of Integrative Agriculture, 2025, 24(1): 246-260.

Wenya Li, Haoxiang Ma, Yanxing Wang, Yushi Zhang, Yang Liu, Ruili Han, Hong Li, Hanfang Cai, Xiaojun Liu, Xiangtao Kang, Ruirui Jiang, Zhuanjian Li. The VGLL2 gene participates in muscle development in Gushi chickens[J]. Journal of Integrative Agriculture, 2025, 24(1): 246-260.

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