Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection

doi:10.1016/j.jia.2023.02.032

摘要/Abstract

Abstract:

A headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS) method was used to study the volatile organic compounds (VOCs) associated with the differential immune response of tomato plants infected with the recombinant strain of potato virus Y (PVY^C-to), necrogenic to tomato. Analysis was carried out in UC82 (UC), a virus susceptible tomato variety, comparing the same UC plants grafted or not onto a virus tolerant tomato ecotype, Manduria (Ma); the three types of samples used for the GC-MS analysis were mock-inoculated UC/Ma plants, UC/Ma+PVY^C-to and UC+PVY^C-to plants; the VOCs obtained were 111. Results from symptomatic PVY^C-to-infected UC plants showed a VOCs composition enriched in alcohols, fatty acid derivates, benzenoids, and salicylic acid derivatives, while in mock-inoculated UC/Ma plants VOCs were mainly characterized by methyl ester compounds. The VOC profile was in line with RNAseq data analyses, denoting that PVY^C-to viral RNA accumulation and disease symptoms induce the specific transcriptional activation of genes involved in VOCs biosynthesis. Furthermore, principal component analysis highlighted that VOCs of PVY^C-to-infected and mock-inoculated grafted plants were much closer each other than that of symptomatic PVY^C-to-infected non-grafted UC plants. These results suggest that VOCs profiles of tomato plants are related to the viral RNA accumulation, disease intensity and graft-derived tolerance to PVY^C-to infection.

Key words: tomato , potyvirus , VOCs , defense , grafted plants

Roberta SPANÒ, Mariarosaria MASTROCHIRICO, Francesco LONGOBARDI, Salvatore CERVELLIERI, Vincenzo LIPPOLIS, Tiziana MASCIA. [J]. Journal of Integrative Agriculture, 2023, 22(8): 2426-2440.

Roberta SPANÒ, Mariarosaria MASTROCHIRICO, Francesco LONGOBARDI, Salvatore CERVELLIERI, Vincenzo LIPPOLIS, Tiziana MASCIA. Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection[J]. Journal of Integrative Agriculture, 2023, 22(8): 2426-2440.

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