小麦抗旱相关基因TaPYL4的功能分析及标记开发

doi:10.1016/S2095-3119(21)63699-7

摘要/Abstract

摘要：

本文研究了TaPYL4在中国小麦种质中的单元型分布及其遗传效应。通过克隆小麦TaPYL4的A、B、D基因组序列并分析差异信息，表明TaPYL4-2A在启动子区含有3种单元型，TaPYL4-2B和TaPYL4-2D含有2种单元型。利用262份中国小麦微核心种质和239份山西省小麦品种(系)进行候选基因关联分析，结果表明：TaPYL4-2A与株高和抗旱系数（DTC）显著相关，PYL4-2A-Hap2是降低株高、增加DTC的优异单元型；TaPYL4-2B与穗粒数显著相关，PYL4-2B-Hap1是增加穗粒数的优异单元型，在育种过程中受到人工选择。

Abstract: ABA receptors (PYR/PYL/RCAR) play a central role in the water loss control of plants. A previous report indicated that TaPYL4 is a critical gene in wheat that improves grain production under drought conditions and increases water use efficiency. In this study, we analyzed the sequence polymorphisms and genetic effects of TaPYL4s. Based on isolated TaPYL4 genes from chromosomes 2A, 2B and 2D, three haplotypes were detected in the promoter region of TaPYL4-2A, and two haplotypes were present in TaPYL4-2B and TaPYL4-2D, respectively. Marker/trait association analysis indicated that TaPYL4-2A was significantly associated with plant height in 262 Chinese wheat core collection accessions, as well as the drought tolerance coefficient (DTC) for plant height in 239 wheat varieties from Shanxi Province in multiple environments. However, the frequencies of favored drought-tolerant haplotype TaPYL4-2A-Hap2 were considerably low, accounting for only 10%, and lines with this certain Hap could be reserved in the breeding program. TaPYL4-2B was significantly associated with grain number, and the favored haplotype TaPYL4-2B-Hap1 was the dominant allele of above 90% in the collection. For TaPYL4-2D, there were no significant differences in these traits between the two haplotypes in either of the two panels. These results indicate that variation might lead to functional differentiation among the homoeologs and the haplotypes had undergone artificial selection during breeding. Two molecular markers developed to distinguish these haplotypes could be used for breeding in water-limited regions.

Key words: TaPYL4 , haplotypes , neo-functionalization , molecular markers

. 小麦抗旱相关基因TaPYL4的功能分析及标记开发[J]. Journal of Integrative Agriculture, 2022, 21(8): 2183-2196.

WU Bang-bang, SHI Meng-meng, Mohammad POURKHEIRANDISH, ZHAO Qi, WANG Ying, YANG Chen-kang, QIAO Ling, ZHAO Jia-jia, YAN Su-xian, ZHENG Xing-wei, ZHENG Jun. Allele mining of wheat ABA receptor at TaPYL4 suggests neo-functionalization among the wheat homoeologs[J]. Journal of Integrative Agriculture, 2022, 21(8): 2183-2196.

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