JIA-2021-0045 QoIs和SDHIs类杀菌剂对禾谷镰刀菌线粒体动态平衡的影响依赖于FgDnm1

doi:10.1016/j.jia.2022.08.118

摘要/Abstract

摘要：

禾谷镰刀菌（Fusarium graminearum）引起的小麦赤霉病（Fusarium head blight, FHB）是一种严重为害粮食作物的真菌病害，不仅引起作物产量损失与品质下降，而且病原菌在感病的谷粒中分泌的真菌毒素，严重威胁粮食安全。前期研究表明甲氧基丙烯酸酯类（quinone outside inhibitors, QoIs）和琥珀酸脱氢酶抑制剂类（succinate dehydrogenase inhibitors, SDHIs）杀菌剂可破坏线粒体动态平衡，引起线粒体碎片化。动力蛋白和动力相关蛋白（DRPs）作为GTPase超家族成员，参与调控真核细胞线粒体分裂、囊泡出芽与分裂等功能，但其在禾谷镰刀菌中的功能尚不清楚。在本研究中，我们利用BLAST分析发现禾谷镰孢菌中与酵母Dnm1的同源蛋白FgDnm1，并对其进行了生物学功能研究。结果表明，FgDnm1参与调控着菌丝生长、有性生殖及杀菌剂药敏性。此外，我们利用荧光标记技术和激光共聚焦显微镜发现FgDnm1与线粒体共定位，且参与调控禾谷镰刀菌产毒小体的结构形成及脱氧雪腐镰刀菌烯醇（DON）的生物合成。进一步研究表明，甲氧基丙烯酸酯类杀菌剂（QoIs）和琥珀酸脱氢酶抑制剂类杀菌剂（SDHIs）均会引起线粒体的碎片化，FgDnm1的缺失会导致线粒体呈现丝状网络分布，并阻断了QoIs和SDHIs诱导的线粒体碎片化。本研究揭示了线粒体动态平衡对禾谷镰刀菌菌丝生长发育、杀菌剂敏感性和毒素形成的影响。因此，我们推论QoIs与SDHIs杀菌剂引起的线粒体动态平衡变化依赖于FgDnm1。

Abstract:

Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating fungal disease on small grain cereal crops, because it reduces yield and quality and causes the mycotoxin contamination to the grain. Dynamins and dynamin-related proteins (DRPs) are large GTPase superfamily members, which are typically involved in the budding and division of vesicles in eukaryotic cells, but their roles in Fusarium spp. remain unexplored. Here, we found that FgDnm1, a DRP and homolog to Dnm1 in Saccharomyces cerevisiae, contributes to the normal fungal growth, sexual reproduction and sensitivity to fungicides. In addition, we found FgDnm1 co-localizes with mitochondria and is involved in toxisome formation and deoxynivalenol (DON) production. Several quinone outside inhibitors (QoIs) and succinate dehydrogenase inhibitors (SDHIs) cause fragmentated morphology of mitochondria. Importantly, the deletion of FgDnm1 displays filamentous mitochondria and blocks the mitochondrial fragmentation induced by QoIs and SDHIs. Taken together, our studies uncover the effect of mitochondrial dynamics in fungal normal growth and how such events link to fungicides sensitivity and toxisome formation. Thus, we concluded that altered mitochondrial morphology induced by QoIs and SDHIs depends on FgDnm1.

Key words: Fusarium graminearum , FgDnm1 , mitochondrial dynamics , fungicides

. JIA-2021-0045 QoIs和SDHIs类杀菌剂对禾谷镰刀菌线粒体动态平衡的影响依赖于FgDnm1[J]. Journal of Integrative Agriculture, 2023, 22(2): 481-494.

KANG Jin-bo, ZHANG Jie, LIU Yin-kai, SONG Ji-chang, OU Jian-lin, TAO Xian, ZHOU Ming-guo, DUAN Ya-bing. Mitochondrial dynamics caused by QoIs and SDHIs fungicides depended on FgDnm1 in Fusarium graminearum[J]. Journal of Integrative Agriculture, 2023, 22(2): 481-494.

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