JIA-2021-1554 羔羊出生至4月龄粪便细菌群落演替过程及其与血清免疫指标的相关性

doi:10.1016/j.jia.2022.08.055

摘要/Abstract

摘要：

微生物在胃肠道发育早期的定植和演替过程对于宿主免疫系统的发育十分重要。本试验以湖羊为研究对象，选择10只初生湖羊母羔（2.87 ± 0.28 kg），利用16S rDNA测序手段，测定0,3,10,20,30,45,60,90,120日龄羔羊粪便细菌区系，探究羔羊从出生至120日龄，体重、血清指标和粪便细菌群落的动态变化，及三者之间的关系。研究结果表明，羔羊生长性能、血清指标、粪便细菌群落和粪便细菌功能均受羔羊日龄的影响(P<0.05)。羔羊平均日增重在60-90日龄时最低(P<0.05)，可能是断奶应激造成。羔羊的免疫反应在30日龄时最高，此时羔羊血清中免疫球蛋白（免疫球蛋白A，免疫球蛋白G，免疫球蛋白M）、细胞因子（白细胞介素-1β，白细胞介素-6，白细胞介素-12、白细胞介素-17、肿瘤坏死因子-α）和肠道通透性指标（D-乳酸和内毒素）水平高于其它日龄组(P<0.05)。各日龄组内粪便细菌群落相似性分析表明，同日龄组内相似性在断奶前较低，在断奶（60日龄）后显著增加(P<0.05)。各日龄与120日龄粪便细菌群落相似性表明，30日龄后，与120日龄的相似性显著增加(P<0.05)。粪便细菌属Lachnospiraceae UCG-010, Eubacterium coprostanoligenes group, Ruminococcaceae UCG-005, Ruminococcaceae UCG-009, Ruminococcaceae UCG-013, Ruminiclostridium 6, Ruminococcaceae UCG-008和Oscillibacter与血清中内毒素、D-乳酸、免疫球蛋白（免疫球蛋白A，免疫球蛋白G，免疫球蛋白M）、细胞免疫因子（白细胞介素-1β，白细胞介素-6，白细胞介素-12和白细胞介素-17）和肿瘤坏死因子-α水平呈负相关(P<0.05)，并且这些属的相对丰度从45日龄时增加。本研究结果表明，与血清免疫指标相关属的相对丰度从45日龄起随羔羊年龄增加，羔羊出生至45日龄可能存在关键调控时期，为调控羔羊微生物以提高羔羊免疫性能提供机会。本研究分析比较了羔羊初生至120日龄，粪便细菌建立过程及其与羔羊体重和血清免疫指标的关系，分析发现了调控羔羊微生物区系的关键时期。

Abstract:

Early bacterial colonization and succession within the gastrointestinal tract have been suggested to be crucial in the development of host immunity. In this study, we have investigated the changes in live weight and concentrations of selected serum parameters in relation to their fecal bacterial communities as determined by high throughput sequencing of the 16S rRNA gene over the same period in lambs. The results showed that lambs’ growth performance, the serum parameters, fecal bacterial community and fecal bacterial functions were all affected (P<0.05) by age of the lambs. Similarity within age groups of fecal microbiota was lower in the preweaning period and increased sharply (P<0.05) after weaning at 60 days. The similarity between the samples collected from birth to 90 days of age and those collected at 120 days of age, increased (P<0.05) sharply after 30 days of age. Some age-associated changes in microbial genera were correlated with the changes in concentrations of immune indicators, including negative (P<0.05) correlations between the relative abundance of Lachnospiraceae UCG-010, Eubacterium coprostanoligenes group, Ruminococcaceae UCG-005, Ruminococcaceae UCG-009, Ruminococcaceae UCG-013, Ruminiclostridium 6, Ruminococcaceae UCG-008, and Oscillibacter with serum concentrations of lipopolysaccharide (LPS), D-lactate dehydrogenase (DLA), immunoglobulin (IgA, IgM, and IgG), and cytokines (interleukin-1β (IL-1β), IL-6, IL-12, and IL-17), tumor necrosis factor-α (TNF-α), and the relative abundance of these genera increased from 45 days of age. In conclusion, these results suggested that the age-related abundances of particular genera were correlated with serum markers of immunity in lambs, and there might be a critical window in the period from birth to 45 days of age which provide an opportunity for potential manipulation of the fecal microbial ecosystems to enhance immune function.

Key words: fecal bacteria , establishment , sheep , serum parameter , early life

. JIA-2021-1554 羔羊出生至4月龄粪便细菌群落演替过程及其与血清免疫指标的相关性[J]. Journal of Integrative Agriculture, 2023, 22(2): 537-550.

YIN Xue-jiao, JI Shou-kun, DUAN Chun-hui, TIAN Pei-zhi, JU Si-si, YAN Hui, ZHANG Ying-jie, LIU Yue-qin. The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months[J]. Journal of Integrative Agriculture, 2023, 22(2): 537-550.

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JIA-2021-1554 羔羊出生至4月龄粪便细菌群落演替过程及其与血清免疫指标的相关性

The succession of fecal bacterial community and its correlation with the changes of serum immune indicators in lambs from birth to 4 months

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