Genetics and fitness costs of resistance to flupyradifurone in Bemisia tabaci from China

doi:10.1016/S2095-3119(20)63500-6

摘要/Abstract

摘要：

氟吡呋喃酮是蔬菜安全生产过程中防治烟粉虱的新型高效杀虫剂。本研究分别利用田间采集的氟吡呋喃酮抗性种群WH-R（抗性水平约199倍）和室内筛选氟吡呋喃酮抗性种群FLU-SEL（抗性水平约124倍）对抗性遗传方式及相关适合度代价进行了阐明。利用室内敏感种群MED-S分别与WH-R和FLU-SEL开展正反交试验，分别获得MED-S与WH-R的后代F_1A、F_1B、F₁以及MED-S与FLU-SEL的后代F_1C、F_1D、F₁'。室内毒理测定试验发现WH-R对氟吡呋喃酮的抗性是常染色体，不完全显性遗传；同时发现FLU-SEL对氟吡呋喃酮的抗性是常染色体，不完全隐形遗传。通过对MED-S、WH-R和FLU-SEL的适合度进行评价发现，WH-R和FLU-SEL在生长发育过程中均存在显著适合度代价。相比于MED-S，WH-R和FLU-SEL的相对适合度为0.50和0.65，并均表现出发育历期延长、卵孵化率下降、若虫和伪蛹期存活率降低；此外，WH-R的成虫产卵量显著下降。本研究相关结果表明抗性相关适合度代价有利于延缓田间烟粉虱对氟吡呋喃酮的抗性进化，并对田间烟粉虱治理策略的制订提供理论支持。

Abstract: Flupyradifurone is a promising new insecticide used for controlling Bemisia tabaci during vegetable production. In this study, we assessed the fitness costs and mode of inheritance associated with resistance to flupyradifurone in B. tabaci by comparing the susceptible strain (MED-S) to one field-evolved flupyradifurone-resistant strain (WH-R, with 199-fold resistance) and one laboratory-selected flupyradifurone-resistant strain (FLU-SEL, with 124-fold resistance). Progenies of reciprocal crosses between WH-R and MED-S (F_1A, F_1B, and pooled F₁), and between FLU-SEL and MED-S (F_1C, F_1D, and pooled F₁’), showed varying degrees of dominance, indicating that resistance to flupyradifurone in WH-R was autosomal and incompletely dominant, yet in FLU-SEL it was autosomal and incompletely recessive. Furthermore, the development of resistance to flupyradifurone occurred at the expense of fitness costs for the resistant populations. Compared to the MED-S strain, WH-R showed a relative fitness of 0.50 with significantly prolonged developmental durations and reduced survival rates of the nymphal and pseudopupal stages, as well as decreased fecundity and hatchability. Similarly, FLU-SEL showed a relative fitness of 0.65 and also demonstrated prolonged developmental durations and reduced survival rates of nymphs and pseudopupae, as well as decreased hatchability in comparison with the MED-S strain. However, no significant differences in fecundity were observed between MED-S and FLU-SEL. The present study provides useful knowledge for formulating pest management strategies in the field, which will allow growers to slow the development of resistance to flupyradifurone and to sustainably control B. tabaci.

Key words: Bemisia tabaci , flupyradifurone , insecticide resistance , inheritance , fitness costs

. [J]. Journal of Integrative Agriculture, 2022, 21(5): 1436-1443.

WANG Ran, ZHANG Jia-song, CHE Wu-nan, WANG Jin-da, LUO Chen . Genetics and fitness costs of resistance to flupyradifurone in Bemisia tabaci from China[J]. Journal of Integrative Agriculture, 2022, 21(5): 1436-1443.

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