JIA-2021-1540

doi:10.1016/j.jia.2022.07.048

摘要/Abstract

摘要：

大豆孢囊线虫（SCN, Heterodera glycines）严重制约大豆生产。大豆抗线虫数量性状遗传位点Rhg4上的丝氨酸羟甲基转移酶编码基因（GmSHMT08）对大豆孢囊线虫有显著的抗性，但该基因如何介导了对大豆孢囊线虫的抗性机制仍不明晰，GmSHMT08能否与大豆孢囊线虫产生的蛋白发生互作仍不明确。本研究以GmSHMT08作为诱饵，通过酵母双杂交体系在线虫中筛选出了与GmSHMT08互作的一个热休克蛋白70片段（HgHSP70p）。通过GST pull-down和荧光双分子互补，进一步验证了HgHSP70p与GmSHMT08之间存在互作关系。本研究发现的HgHSP70基因可以作为关键候选基因，用于进一步探究GmSHMT08介导的对大豆孢囊线虫的抗性机制。

Abstract:

Soybean cyst nematode (SCN) Heterodera glycines is considered as the major constraint to soybean production. GmSHMT08 at Rhg4 locus on chromosome 08, encoding a serine hydroxylmethyltransferase, is a major gene underlying resistance against H. glycines in Peking-type soybeans. However, the molecular mechanism underpinning this resistance is less well characterized, and whether GmSHMT08 could interact with proteins in H. glycines remains unclear. In this study, yeast two-hybrid screening was conducted using GmSHMT08 as a bait protein, and a fragment of a 70-kDa heat shock protein (HgHSP70) was screened from H. glycines that exhibited interaction with GmSHMT08. This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays. Our finding reveals HgHSP70 could be applied as a potential candidate gene for further exploring the mechanism on GmSHMT08-mediated resistance against SCN H. glycines.

Key words: soybean cyst nematode , Heterodera glycines , GmSHMT08 , HgHSP70 , interaction , resistance

. JIA-2021-1540 [J]. Journal of Integrative Agriculture, 2022, 21(10): 2973-2983.

LIU Zhi, ZHANG Liu-ping, ZHAO Jie, JIAN Jin-zhuo, PENG Huan, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming. A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08[J]. Journal of Integrative Agriculture, 2022, 21(10): 2973-2983.

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JIA-2021-1540

A fragment of a 70-kDa Heterodera glycines heat shock protein (HgHSP70) interacts with soybean cyst nematode-resistant protein GmSHMT08

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