JIA-2021-1061  复方中草药制剂对鸡毒支原体诱导的呼吸道炎症的拮抗作用

doi:10.1016/j.jia.2022.07.038

摘要/Abstract

摘要：

鸡毒支原体（Mycoplasma gallisepticum，MG）是引起鸡慢性呼吸道病（Chronic respiratory disease，CRD）的病原体，通过自身黏附蛋白吸附于宿主呼吸道上皮细胞，造成炎症损伤。MG 感染率极高，且常与其他病原微生物混合或继发感染，给家禽业造成巨大的经济损失。目前主要使用抗生素防治 MG 的感染，但抗生素的长期使用和滥用，极易引起大量耐药菌株产生，且造成产品中药物残留，严重威胁人类健康。近年来，新兴的中草药(CHM)被用于治疗CRD。中草药具有清热解毒、抗菌、提高机体抵抗力、毒副作用小以及不产生耐药性的独特优势，在临床实践中有很好的疗效。本试验使用的复方中药制剂(CHMF)由黄芩、鱼腥草、甘草等 10 种中草药组成。试验组共分 10 组，分别为：空白对照组，生理盐水对照组，MG感染组，泰妙菌素治疗组，中药预防和治疗高（1 g d^-1）、中（0.75 g d^-1）、低（0.5 g d^-1）剂量组。通过体外抑菌试验和 MG 感染的防治试验确定其防治 CRD 的有效性，并在一定程度上探究其作用机制，为中药复方制剂防治 CRD 提供理论依据。在预防效果方面，CHMF能有效减轻MG引起的体重和饲料系数下降。组织病理学切片结果表明，预防和治疗CHMF均能明显缓解MG感染引起的严重呼吸道炎症。此外，与MG感染组相比，CHMF预防组和治疗组均能有效降低MG粘附蛋白(pMGA1.2)的表达，抑制MG的增殖和黏附，从而有效抑制MG诱导的炎症因子白介素1β (IL-1β)和肿瘤坏死因子-α (TNF-α)的表达。综上所述，上述结果证实了CHMF能够保护雏鸡免受MG感染引起的组织损伤，且在短期内对雏鸡生产性能无不良影响。并且我们还发现CHMF对MG的疗效与泰妙菌素相当或优于泰妙菌素。综合本研究结果，该复方中药通过直接抑制或杀灭 MG，阻断MG的黏附，能有效缓解由 MG感染引起的生产性能下降，抑制 MG 感染引起的炎症反应。推荐该中药制剂预防和治疗 MG 感染的剂量为 1g d^-1，连用 7d。

Abstract:

Mycoplasma gallisepticum HS strain (MG-HS) is a pathogen that causes chronic respiratory disease (CRD) in chicken, which is characterized by host respiratory inflammatory damage, brings huge economic losses to the poultry industry. Recently, emerging Chinese herbal medicines (CHM) have been used to treat CRD. This study was aimed to investigate the preventive and therapeutic effects and their potential mechanisms of Chinese herbal medicinal formulae (CHMF), which consisted of 10 kinds of Chinese herbal medicine including Scutellaria, Houttuynia cordate and licorice, on MG-induced CRD in chickens. With respect to the preventive effect, the results showed that CHMF could effectively recover the MG-induced decrease on body weight and feed conversion ratio. Histopathological analysis showed that both prevention and treatment of CHMF could significantly alleviate the severe respiratory inflammation induced by MG infection. Moreover, compared with the MG infection group, both the prevention groups and the treatment groups of CHMF could effectively reduce the expression of MG adhesion protein (pMGA1.2) to inhibit the proliferation of MG, and thus effectively inhibit the expression of MG-induced inflammatory factors interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α). In summary, these findings confirm that CHMF can protect chickens from various tissue damage caused by MG infection and has no adverse effects on the performance of chickens in the short term. And its efficacy against MG is equal to or better than that of tiamulin.

Key words: Mycoplasma gallisepticum HS strain (MG-HS) , Chinese herbal medicine formulae , production performance , inflammatory lesions , chicken

. JIA-2021-1061 复方中草药制剂对鸡毒支原体诱导的呼吸道炎症的拮抗作用[J]. Journal of Integrative Agriculture, 2022, 21(10): 3026-3036.

WANG Ying-jie, LIANG Ya-xi, HU Fu-li, SUN Ying-fei, ZOU Meng-yun, LUO Rong-long, PENG Xiu-li. Chinese herbal formulae defend against Mycoplasma gallisepticum infection[J]. Journal of Integrative Agriculture, 2022, 21(10): 3026-3036.

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JIA-2021-1061 复方中草药制剂对鸡毒支原体诱导的呼吸道炎症的拮抗作用

Chinese herbal formulae defend against Mycoplasma gallisepticum infection

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