Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

doi:10.1016/S2095-3119(21)63696-1

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (2): 586-595.DOI: 10.1016/S2095-3119(21)63696-1

收稿日期:2020-10-06 接受日期:2021-03-22 出版日期:2022-01-02 发布日期:2022-01-02

Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

<a href="https://www.chinaagrisci.com/Jwk_zgnykxen/EN/article/advancedSearchResult.do?searchSQL=(((Sulaiman ABDULSALAM[Author]) AND 1[Journal]) AND year[Order])" target="_blank">Sulaiman ABDULSALAM</a>1, 2, PENG Huan1, LIU Shi-ming1, HUANG Wen-kun1, KONG Ling-an1, PENG De-liang1

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² Division of Agricultural Colleges, Department of Crop Protection, Ahmadu Bello University, Zaria 810107, Nigeria

Received:2020-10-06 Accepted:2021-03-22 Online:2022-01-02 Published:2022-01-02
About author:Sulaiman ABDULSALAM, E-mail: asulaiman@abu.edu.ng; Correspondence PENG De-liang, E-mail: dlpeng@ippcaas.cn
Supported by:
This work was funded by the National Natural Science Foundation of China (31772142), the Research Foundation for Ph D of the Graduate School of Chinese Academy of Agricultural Sciences, China (2018Y90100033), and the Special Fund for Agro-Scientific Research in the Public Interest of China (210503114).

摘要/Abstract

Abstract: Stunt nematodes (Tylenchorhynchus spp.) are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated crops. These nematodes, with phyto-sanitary potential, are frequently ignored or misdiagnosed as pests, and this may pose a threat to food security. The accuracy of its identification based on a morphological approach has been challenged recently, due to the overlapping of the morphological and morphometric characters of the species. Consequently, the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops (wheat, maize and rice) in the savannahs of northern Nigeria, using integrative taxonomy and molecular approaches. The molecular and morphological studies identified and confirmed the presence of T. annulatus as the occurring specie in the savannahs of northern Nigeria. The phylogenetic analysis was carried out using the internal transcribed spacer (ITS) and 28S genes of ribosomal DNA further confirmed the presence of T. annulatus. The first molecular characterization and sequences of the ITS and 28S rDNA gene for T. annulatus from Nigeria were provided by this research. Also, according to our literature search, this is the first report on T. annulatus in wheat, maize and rice in the savannahs of northern Nigeria. Further study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies.

Key words: plant-parasitic nematodes , food security , Tylenchorhynchus annulatus , ITS , phylogeny

. [J]. Journal of Integrative Agriculture, 2022, 21(2): 586-595.

Sulaiman ABDULSALAM, , PENG Huan, LIU Shi-ming, HUANG Wen-kun, KONG Ling-an, PENG De-liang . Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria[J]. Journal of Integrative Agriculture, 2022, 21(2): 586-595.

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Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

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