JIA-2021-1575 分子标记辅助选择聚合赤霉病抗性位点Fhb1和Fhb2以改良优质弱筋小麦品种扬麦15

doi:10.1016/j.jia.2022.08.057

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (2): 360-370.DOI: 10.1016/j.jia.2022.08.057

JIA-2021-1575 分子标记辅助选择聚合赤霉病抗性位点Fhb1和Fhb2以改良优质弱筋小麦品种扬麦15

收稿日期:2021-09-03 接受日期:2021-11-23 出版日期:2023-02-20 发布日期:2021-11-23

Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in a high-quality soft wheat cultivar Yangmai 15

HU Wen-jing^{1, 2}, FU Lu-ping^{3, 4}, GAO De-rong^{1, 2}, LI Dong-sheng¹, LIAO Sen^{1, 5}, LU Cheng-bin^{1, 2}

¹Key Laboratory of Wheat Biology and Genetic Improvement for Low & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs, Lixiahe Institute of Agricultural Sciences, Yangzhou 225007, P.R.China
²College of Agronomy & Center for Crop Genome Engineering, Henan Agricultural University, Zhengzhou 45002, P.R.China
³Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College, Yangzhou University, Yangzhou 225009, P.R.China
⁴Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
⁵College of Agriculture, Yangtze University, Jingzhou 434023, P.R.China

Received:2021-09-03 Accepted:2021-11-23 Online:2023-02-20 Published:2021-11-23
About author:HU Wen-jing, E-mail: huren2008@126.com; Correspondence LU Cheng-bin, Tel: +86-514-87303751, Fax: +86-514-87307821 E-mail: lucb123@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China (31901544 and 2071999) and the National Key Research and Development Program of China (2017YFD0100801).

摘要/Abstract

摘要： 赤霉病(Fusarium head blight, FHB)是严重影响小麦产量和品质的主要病害之一，在我国长江中下游地区尤为严重。Fhb1和Fhb2是两个主要抗赤霉病位点。扬麦15 (YM 15) 弱筋品质好，是长江中下游地区最受欢迎的品种之一，但对赤霉病抗性较差。本研究采用分子标记辅助选择(MAS)回交策略，利用Fhb1和Fhb2提高扬麦15的赤霉病抗性。每世代进行农艺性状的筛选。我们成功地选择了7个同时携带Fhb1和Fhb2纯合基因型的渐渗系，它们的赤霉病抗性显著高于背景亲本扬麦15。其中3个渐渗系的农艺性状和品质性状与扬麦15相似。本研究表明，利用分子标记聚合Fhb1和Fhb2可显著提高小麦对赤霉病的抗性。

Abstract:

Fusarium head blight (FHB) is one of the most detrimental wheat diseases which greatly decreases the yield and grain quality, especially in the middle and lower reaches of the Yangtze River of China. Fhb1 and Fhb2 are two major resistance loci against Fusarium graminearum. Yangmai 15 (YM15) is one of the most popular varieties in the middle and lower reaches of the Yangtze River, and it has good weak gluten characters but poor resistance to FHB. Here we used Fhb1 and Fhb2 to improve the FHB resistance of YM15 by a molecular marker-assisted selection (MAS) backcrossing strategy. The selection of agronomic traits was performed for each generation. We successfully selected seven introgressed lines which carry homozygous Fhb1 and Fhb2 with significantly higher FHB resistance than the recurrent parent YM15. Three of the introgressed lines had agronomic and quality characters that were similar to YM15. This study demonstrates that the pyramiding of Fhb1 and Fhb2 could significantly improve the FHB resistance in wheat using the MAS approach

Key words: Fusarium head blight , marker-assisted selection , Fhb1 , Fhb2 , resistance breeding , wheat

. JIA-2021-1575 分子标记辅助选择聚合赤霉病抗性位点Fhb1和Fhb2以改良优质弱筋小麦品种扬麦15[J]. Journal of Integrative Agriculture, 2023, 22(2): 360-370.

HU Wen-jing, FU Lu-ping, GAO De-rong, LI Dong-sheng, LIAO Sen, LU Cheng-bin. Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in a high-quality soft wheat cultivar Yangmai 15[J]. Journal of Integrative Agriculture, 2023, 22(2): 360-370.

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JIA-2021-1575 分子标记辅助选择聚合赤霉病抗性位点Fhb1和Fhb2以改良优质弱筋小麦品种扬麦15

Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in a high-quality soft wheat cultivar Yangmai 15

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