Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (11): 2746-2757.DOI: 10.1016/S2095-3119(20)63226-9

所属专题: 昆虫合辑Plant Protection—Entomolgy 昆虫分子生物学合辑Insect Molecular Biology 昆虫和植物互作合辑Insect and Plant Interact

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  • 收稿日期:2019-09-30 出版日期:2020-11-01 发布日期:2020-10-15

Molecular characteristics and temperature tolerance function of the transient receptor potential in the native Bemisia tabaci AsiaII3 cryptic species

JI Shun-xia1, SHEN Xiao-na1, LIANG Lin1, WANG Xiao-di1, LIU Wan-xue1, WAN Fang-hao1, 2, LÜ Zhi-chuang1 
  

  1. 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
    2 Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
  • Received:2019-09-30 Online:2020-11-01 Published:2020-10-15
  • Contact: Correspondence Lü Zhi-chuang, Tel/Fax: +86-10-82109572, E-mail: lvzhichuang@caas.cn
  • About author:JI Shun-xia, E-mail: 82101172334@caas.cn;
  • Supported by:
    This work was funded by the National Natural Science Foundation of China (31672088 and 31601695), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (caascx-2017-2022-IAS) and the National Key Research and Development Program of China (2016YFC1200603).

Abstract:

Insects are poikilothermic animals, and temperature is one of the most important abiotic factors affecting their spread and distribution.  For example, differences in thermal tolerance may underlie the significant differences in geographical distributions between the native AsiaII3 and invasive MED (Mediterranean) cryptic Bemisia tabaci species in China.  Transient receptor potential (TRP) channels are key components of the insect temperature perception system and act as molecular thermometers since they can be activated by specific changes in temperature.  In this study, we cloned and characterized the AsiaII3 BtTRP gene and revealed its functions in the response to thermal stress.  The full-length cDNA of BtTRP was 3 821 bp, with a 3 501-bp open reading frame encoding a 132.05-kDa protein.  Comparing the deduced amino acid sequences of AsiaII3 BtTRP and MED TRP revealed five amino acid differences.  In situ hybridization indicated that BtTRP might be widely expressed throughout the AsiaII3 adult body.  BtTRP mRNA expression reached the highest levels after exposure to mild thermal stimuli (12 and 35°C), showing that BtTRP expression can be induced by temperature stress.  Furthermore, the thermal tolerance of AsiaII3 after BtTRP dsRNA feeding was significantly lower than that of the control.  Taken together, the present study highlights the importance of TRP channels for B.?tabaci thermal resistance, and allows us to infer that the differences in amino acids between AsiaII3 and MED might cause the differences in thermal tolerance of these two cryptic species.  This study provides a new direction for investigating geographic distribution differences between invasive and native insects.

Key words: transient receptor potential ,  thermosensation ,  thermal stress ,  Bemisia tabaci , AsiaII3