Identification of main effect and epistatic QTLs controlling initial flowering date in cultivated peanut (Arachis hypogaea L.)

doi:10.1016/S2095-3119(20)63211-7

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (10): 2383-2393.DOI: 10.1016/S2095-3119(20)63211-7

收稿日期:2019-07-04 出版日期:2020-10-01 发布日期:2020-09-08

Identification of main effect and epistatic QTLs controlling initial flowering date in cultivated peanut (Arachis hypogaea L.)

WANG Liang^{1, 2, 3}, YANG Xin-lei¹, CUI Shun-li¹, WANG Ji-hong^{4, 5}, HOU Ming-yu¹, MU Guo-jun¹, LI Zi-chao², LIU Li-feng¹

¹ College of Agronomy, Hebei Agricultural University/North China Key Laboratory for Crop Germplasm Resources, Ministry of Education/State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071001, P.R.China
² College of Agronomy and Biotechnology, China Agricultural University/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193, P.R.China
³ Crop Research Institute, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, P.R.China
⁴ School of Statistics, University of International Business and Economics, Beijing 100029, P.R.China
⁵ College of Science, Shihezi University, Shihezi 832003, P.R.China

Received:2019-07-04 Online:2020-10-01 Published:2020-09-08
Contact: Correspondence LIU Li-feng, Tel: +86-312-7528136, E-mail: lifengliucau@126.com; LI Zi-chao, Tel: +86-10-62731414, E-mail: lizichao@cau.edu.cn
About author:WANG Liang, Tel: +86-312-7528136, E-mail: nkywangliang@163.com;
Supported by:
This study was supported by the earmarked fund for China Agriculture Research System (CARS-13), the National Natural Science Foundation of China (31771833), the Science and Technology Supporting Plan Project of Hebei Province, China (16226301D), and the Key Projects of Science and Technology Research in Higher Education Institution of Hebei Province, China (ZD2015056).

摘要/Abstract

Abstract:

Initial flowering date (IFD) is closely related to mature period of peanut pods. In present study, a population of recombinant inbred lines (RIL) derived from the cross between Silihong (female parent) and Jinonghei 3 (male parent) was used to map QTLs associated with IFD. The RIL population and its two parental cultivars were planted in two locations of Hebei Province, China from 2015 to 2018 (eight environments). Based on a high-density genetic linkage map (including 2 996 SNP and 330 SSR markers) previously constructed in our laboratory, QTLs were analyzed using phenotypic data and the best linear unbiased prediction (BLUP) value of initial flowering date by inclusive composite interval mapping (ICIM) method. Interaction effects between every two QTLs and between individual QTL and environment were also analyzed. In cultivated peanut, IFD was affected by genotypic factor and environments simultaneously, and its broad sense heritability (h2) was estimated as 86.8%. Using the IFD phenotypic data from the eight environments, a total of 19 QTLs for IFD were detected, and the phenotypic variation explained (PVE) by each QTL ranged from 1.15 to 21.82%. Especially, five of them were also detected by the BLUP value of IFD. In addition, 12 additive QTLs and 35 pairs of epistatic QTLs (62 loci involved) were identified by the joint analysis of IFD across eight environments. Three QTLs (qIFDB04.1, qIFDB07.1 and qIFDB08.1) located on chromosome B04, B07 and B08 were identified as main-effect QTL for IFD, which had the most potential to be used in peanut breeding. This study would be helpful for the early-maturity and adaptability breeding in cultivated peanut.

Key words: peanut (Arachis hypogaea L.) , initial flowering date (IFD) , QTL , best linear unbiased prediction (BLUP) , ICIM

. [J]. Journal of Integrative Agriculture, 2020, 19(10): 2383-2393.

WANG Liang, YANG Xin-lei, CUI Shun-li, WANG Ji-hong, HOU Ming-yu, MU Guo-jun, LI Zi-chao, LIU Li-feng. Identification of main effect and epistatic QTLs controlling initial flowering date in cultivated peanut (Arachis hypogaea L.)[J]. Journal of Integrative Agriculture, 2020, 19(10): 2383-2393.

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Identification of main effect and epistatic QTLs controlling initial flowering date in cultivated peanut (Arachis hypogaea L.)

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