Effects of different rotation patterns on the occurrence of clubroot disease and diversity of rhizosphere microbes

doi:10.1016/S2095-3119(20)63186-0

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (9): 2265-2273.DOI: 10.1016/S2095-3119(20)63186-0

收稿日期:2019-10-15 出版日期:2020-09-01 发布日期:2020-08-04

Effects of different rotation patterns on the occurrence of clubroot disease and diversity of rhizosphere microbes

YANG Xiao-xiang^{1, 2}, HUANG Xiao-qin^{1, 2}, WU Wen-xian¹, XIANG Yun-jia¹, DU Lei¹, ZHANG Lei^{1, 2}, LIU Yong^{1, 2}

¹ Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
² Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture and Rural Affairs, Chengdu 610066, P.R.China

Received:2019-10-15 Online:2020-09-01 Published:2020-08-04
Contact: Correspondence ZHANG Lei, Tel/Fax: +86-28-84504089, E-mail: zhanglei9296@aliyun.com; LIU Yong, Tel/Fax: +86-28-84504089, E-mail: liuyongdr@163.com
About author:YANG Xiao-xiang, E-mail: yxxsaas@163.com;
Supported by:
This work was supported by the National Key Research and Development Program of China (2017YFD0200600), the Financial Innovation Capacity Enhancement Project in Sichuan Province, China (2019QNJJ-011), and the National Modern Agricultural Industry Technology System of Sichuan Rape Innovation Team, China (2019–2023).

摘要/Abstract

Abstract:

Clubroot disease, caused by Plasmodiophora brassicae, is one of the most destructive soil-borne diseases in cruciferous crops worldwide. New strategies are urgently needed to control this disease, as no effective disease-resistant varieties or chemical control agents exist. Previously, we found that the incidence rate and disease index of clubroot in oilseed rape decreased by 50 and 40%, respectively, when oilseed rape was planted after soybean. In order to understand how different rotation patterns affect the occurrence of clubroot in oilseed rape, high-throughput sequencing was used to analyze the rhizosphere microbial community of oilseed rape planted after leguminous (soybean, clover), gramineous (rice, maize) and cruciferous (oilseed rape, Chinese cabbage) crops. Results showed that planting soybeans before oilseed rape significantly increased the population density of microbes that could inhibit P. brassicae (e.g., Sphingomonas, Bacillus, Streptomyces and Trichoderma). Conversely, consecutive cultivation of cruciferous crops significantly accumulated plant pathogens, including P. brassicae, Olpidium and Colletotrichum (P<0.05). These results will help to develop the most effective rotation pattern for reducing clubroot damage.

Key words: oilseed rape , clubroot , Gramineae , Leguminosae , Cruciferae , rhizosphere soil

. [J]. Journal of Integrative Agriculture, 2020, 19(9): 2265-2273.

YANG Xiao-xiang, HUANG Xiao-qin, WU Wen-xian, XIANG Yun-jia, DU Lei, ZHANG Lei, LIU Yong. Effects of different rotation patterns on the occurrence of clubroot disease and diversity of rhizosphere microbes[J]. Journal of Integrative Agriculture, 2020, 19(9): 2265-2273.

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Effects of different rotation patterns on the occurrence of clubroot disease and diversity of rhizosphere microbes

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