BmApontic is involved in neurodevelopment in the silkworm Bombyx mori

doi:10.1016/S2095-3119(19)62787-5

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (6): 1439-1446.DOI: 10.1016/S2095-3119(19)62787-5

收稿日期:2019-05-09 出版日期:2020-06-01 发布日期:2020-04-26

BmApontic is involved in neurodevelopment in the silkworm Bombyx mori

MENG Miao^1*, YU Qi^{1, 2*,} WANG Qin¹, LIU Chun³, LIU Zhao-yang¹, REN Chun-jiu¹, CUI Wei-zheng¹, LIU Qing-xin¹

¹ Laboratory of Developmental Genetics, Shandong Agricultural University, Tai’an 271018, P.R.China
² Shandong Agricultural University Library, Tai’an 271018, P.R.China
³ State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, P.R.China

Received:2019-05-09 Online:2020-06-01 Published:2020-04-26
Contact: Correspondence LIU Qing-xin, Tel: +86-538-8246398, E-mail: liuqingxin@sdau.edu.cn
About author:MENG Miao, E-mail: 1157744045@qq.com; YU Qi, E-mail: yuqsdau@163.com; * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31571502, 31872971 and 31602011), the Funds of “Shandong Double Tops” Program, China (SYL2017YSTD09) and the earmarked fund for China Agriculture Research System (CARS-22).

摘要/Abstract

Abstract:

The nervous system of the silkworm is vital for the development of organisms. It achieves and maintains normal life activities by regulating the function of the organs and all kinds of physiological processes in the silkworm. BmApontic (BmApt), as an important bZIP transcription factor, is required for the formation of pigmentation in the silkworm. However, the function of BmApt in the development of the nervous system of the silkworm remains unclear. Here, we showed that amino acid sequence of BmApt was evolutionarily conserved in its Myb/SANT motif and basic DNA binding domain. BmApt was expressed in the nervous system at the embryonic stage. Knockdown of Bmapt by RNA interference resulted in abnormal development of axons. Moreover, the expression of BmnetrinA, BmnetrinB and Bmfrazzled was decreased in the Bmapt knockdown embryos. These results demonstrate that BmApt controls neurodevelopment by activating the expression of Bmnetrin and Bmfrazzled.

Key words: Bombyx mori , BmApontic , Bmnetrin , Bmfrazzled , neurodevelopment , RNA interference

. [J]. Journal of Integrative Agriculture, 2020, 19(6): 1439-1446.

MENG Miao, YU Qi, WANG Qin, LIU Chun, LIU Zhao-yang, REN Chun-jiu, CUI Wei-zheng, LIU Qing-xin. BmApontic is involved in neurodevelopment in the silkworm Bombyx mori[J]. Journal of Integrative Agriculture, 2020, 19(6): 1439-1446.

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BmApontic is involved in neurodevelopment in the silkworm Bombyx mori

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