Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

doi:10.1016/S2095-3119(19)62739-5

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (11): 2598-2604.DOI: 10.1016/S2095-3119(19)62739-5

收稿日期:2018-12-29 出版日期:2019-11-01 发布日期:2019-11-02

Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

ZHANG Jun-qin^{1, 2*}, WEI Yan-ming^{1, 2, 3*}, HUANG Kun^{1, 2}, SUN Xiao-mei^{1, 2, 3}, ZOU Zhong^{1, 2, 3}, JIN Mei-lin^{1, 2, 3}

¹ State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P.R.China
² College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, P.R.China
³ Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, P.R.China

Received:2018-12-29 Online:2019-11-01 Published:2019-11-02
Contact: Correspondence JIN Mei-lin, E-mail: jml8328@126.com; ZOU Zhong, E-mail: zz19841024@126.com
About author:* These authors contributed equally to this study.
Supported by:
This research was supported by the National Key Research and Development Program of China (2016YFD0500801).

摘要/Abstract

Abstract:

Hepatitis-hydropericardium syndrome (HHS) is an infectious disease caused by fowl adenovirus serotype 4 (FAdV-4). Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines. However, the protective efficiency of baculovirus-expressed penton base protein has not been assessed. In this study, two recombinant capsid proteins, penton base and fiber-2, were constructed. And then, penton base and fiber-2 were administrated alone or together to specific pathogen-free (SPF) chickens at 14 days of life and boosted at 28 days of life. At 42 days of life, the immunized groups and the control group were challenged with FAdV-4 virulent strain. Results show that inoculating penton base or penton base+fiber-2 provided 100% protection to the chickens. All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions. Thus, baculovirus-expressed penton base protein is a promising candidate subunit vaccine.

Key words: fowl adenovirus serotype 4 , hepatitis-hydropericardium syndrome , penton base , subunit vaccine , baculovirus expression system

. [J]. Journal of Integrative Agriculture, 2019, 18(11): 2598-2604.

ZHANG Jun-qin, WEI Yan-ming, HUANG Kun, SUN Xiao-mei, ZOU Zhong, JIN Mei-lin .

Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

[J]. Journal of Integrative Agriculture, 2019, 18(11): 2598-2604.

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Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome

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