Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation

doi:10.1016/S2095-3119(21)63606-7

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (10): 2749-2757.DOI: 10.1016/S2095-3119(21)63606-7

所属专题：动物科学合辑Animal Science

收稿日期:2020-02-04 出版日期:2021-10-01 发布日期:2021-08-09

Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation

BI Yu-lin^{1, 2*}, YANG Shu-yan^3*, WANG Hai-yan^4*, CHANG Guo-bin¹, CHEN Guo-hong¹

¹ College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China
² Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China
³ Department of Biochemistry, Capital Institute of Pediatrics, Beijing 100020, P.R.China
⁴ Aquacultural College, Rizhao Polytechnic, Rizhao 276826, P.R.China

Received:2020-02-04 Online:2021-10-01 Published:2021-08-09
Contact: Correspondence CHEN Guo-hong, Tel: +86-514-87971833, E-mail: ghchen@yzu.edu.cn; CHANG Guo-bin, Tel: +86-514-87971833, E-mail: passioncgb@163.com
About author:BI Yu-lin, Tel: +86-514-87971833, E-mail: ylbi@yzu.edu.cn; * These authors contributed equally to this study.
Supported by:
This work was funded by the National Natural Science Foundation of China (31802057), the Second Batch of Special Grant from China Postdoctoral Science Foundation (2020TQ0252), the Postdoctoral Research Funding Project of Jiangsu Province, China in 2020 (2020Z213), and the Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China (JILAR-KF202017).

摘要/Abstract

摘要：

卵泡刺激素（FSH）是一种重要的下丘脑-垂体-性腺轴（HPG）激素，由垂体分泌。本研究证实FSH在鸡卵泡不同时期均有表达，颗粒细胞中FSHβ mRNA表达信号强于卵母细胞（P<0.05）。卵巢中FSHβ 369 bp的编码序列与垂体中FSHβ的编码序列完全相同。体外实验表明卵巢具有FSH分泌功能。此外，卵泡中FSHβ mRNA表达显著上调（P<0.05），且随着卵泡发育，FSHβ mRNA表达水平显著高于垂体（P<0.05），约为垂体的2~23倍。siRNA处理后卵巢颗粒细胞数量明显减少（P<0.05），说明卵巢FSH可促进颗粒细胞增殖。这一观点得到了细胞周期分析和CCND2和CCNE2表达的支持。进一步研究表明，经FSHβ siRNA处理的颗粒细胞数与外源性FSH处理的颗粒细胞数无显著差异（P>0.05），而未经FSHβ siRNA转染的颗粒细胞数明显高于外源性FSH处理的颗粒细胞数（P<0.05）。提示外源性FSH对卵巢颗粒细胞的增殖作用依赖于内源性FSH。本研究表明FSH基因在鸡卵泡中表达，促进卵巢颗粒细胞增殖。本研究可以丰富HPG轴的理论。

Abstract:

Follicle-stimulating hormone (FSH), an important hypothalamic-pituitary-gonadal axis (HPG) hormone, is secreted by the pituitary gland. This study confirms that FSH is expressed in chicken follicles at different stages, and positive FSHβ mRNA signals were stronger (P<0.05) in granulosa cells than in oocytes. The 369 bp coding sequence of FSHβ in ovaries is 100% identical to that in the pituitary gland. The experiment in vitro revealed that the ovary possessed FSH secretory capacity. Further, FSHβ mRNA was significantly upregulated (P<0.05) in follicles and significantly higher (P<0.05) than that in the pituitary gland by approximately 2–23 times with the development. The number of granulosa cells decreased significantly (P<0.05) in the cells with siRNA treatment, confirming that the ovarian FSH could promote granulosa cell proliferation. This view was supported by cell cycle analysis and CCND2 and CCNE2 expression. Further research indicated that no difference (P>0.05) was observed between the number of granulosa cells treated with FSHβ siRNA and in exogenous FSH. However, the number of granulosa cells without FSHβ siRNA transfection was significantly higher (P<0.05) for exogenous FSH. This finding suggests that the proliferative effect of exogenous FSH on ovarian granulosa cells depend on endogenous FSH. This study demonstrated that the FSH gene was expressed in chicken follicles and promoted ovarian granulosa cell proliferation, which enriched the theory on HPG axis.

Key words: FSH , expression , follicle , granulosa cell proliferation , chicken

. [J]. Journal of Integrative Agriculture, 2021, 20(10): 2749-2757.

BI Yu-lin, YANG Shu-yan, WANG Hai-yan, CHANG Guo-bin, CHEN Guo-hong. Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation[J]. Journal of Integrative Agriculture, 2021, 20(10): 2749-2757.

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Follicle-stimulating hormone is expressed in ovarian follicles of chickens and promotes ovarian granulosa cell proliferation

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