JIA-2021-0706 危害我国新疆甜菜的孢囊线虫种类首次鉴定

doi:10.1016/S2095-3119(21)63797-8

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (6): 1694-1702.DOI: 10.1016/S2095-3119(21)63797-8

所属专题：线虫合辑Nematology

JIA-2021-0706 危害我国新疆甜菜的孢囊线虫种类首次鉴定

收稿日期:2021-04-20 接受日期:2021-06-28 出版日期:2022-06-01 发布日期:2021-06-28

Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China

PENG Huan^{1, 3}, LIU Hui², GAO Li¹, JIANG Ru¹, LI Guang-kuo³, GAO Hai-feng³, WU Wei⁴, WANG Jun⁵, ZHANG Yu⁵, HUANG Wen-kun¹, KONG Ling-an¹, PENG De-liang¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
² The National Agro-Tech Extension and Service Center, Beijing 100026, P.R.China
³Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, P.R.China
⁴ Agricultural Technology Extension Service Center of Xinyuan County, Yining 835800, P.R.China
⁵ Plant Protection Station of Xinjiang Uygur Autonomous Region, Urumqi 830006, P.R.China

Received:2021-04-20 Accepted:2021-06-28 Online:2022-06-01 Published:2021-06-28
About author:PENG Huan, E-mail: penghuan@caas.cn; Correspondence PENG De-liang, E-mail: pengdeliang@caas.cn
Supported by:
This research was financially supported by the National Natural Science Foundation of China (31972247), the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (ASTIP-02-IPP-15), the Open Fund of Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs (KFJJ202101), and the China Agriculture Research System of MOF and MARA (CARS170307).

摘要/Abstract

摘要：

为明确我国主要甜菜产区线虫的种类和危害程度，2015-2018年我们对我国甜菜主要产区进行了系统的调查和检测。在新疆伊犁州新源县的甜菜地调查发现，部分区域甜菜长势弱，植株黄化、矮化明显，受害植株根系有明显的须根团，根上有大量的白色雌虫。采用形态学和形态测量学的方法对孢囊线虫的雌虫、孢囊和二龄幼虫进行显微观察和测量，结果表明，在我国新疆新源县甜菜上发现的孢囊群体的2龄幼虫、孢囊及阴门锥的特征和关键形态测量值与国外已报道的甜菜孢囊线虫基本一致。采用分子生物学的方法对孢囊线虫新疆新源群体的核糖体DNA-ITS、28S-D2/D3和线粒体DNA COI基因进行克隆、测序和分析发现，新源群体的核糖体DNA-ITS, 28S-D2/D3 和线粒体DNA COI基因序列和国外已报道的甜菜孢囊线虫的一致性为99.81-100%，进化分析显示新疆新源群体与国外甜菜孢囊线虫群体聚类为一个进化分支，同时采用甜菜孢囊线虫特异性引物SHF6和rDNA2，从新疆新源群体中扩增出长度为255 bp的特异性条带，分子生物学检测结果表明：我国新疆甜菜上发现的孢囊线虫为甜菜孢囊线虫。采用柯赫氏法则，将500头二龄幼虫分别接种到甜菜（SD21816）和油菜（德油6号）根系中，15天后在甜菜和油菜根系上分别发现了137和157个孢囊和雌虫，表明新疆新源群体能够在甜菜和油菜上完成生活史，且形态学和分子生物学鉴定结果和田间样品完全一致。综上所述，经形态学鉴定和分子特征分析确认，在我国新疆新源县甜菜上发现的孢囊线虫为我国检疫性有害生物——甜菜孢囊线虫，这也是甜菜孢囊线虫在我国的首次明确报道。

Abstract: The sugar beet cyst nematode, Heterodera schachtii, is a major parasite of sugar beet which has been recognized and listed as a quarantine nematode in China and more than 20 countries and regions worldwide. A survey for important nematodes was undertaken in the sugar beet planting area of China during 2015–2018, and numerous cysts were collected from sugar beet fields in Xinyuan County, Xinjiang Uygur Autonomous Region of China. The observations of morphological and morphometric characteristics revealed that cysts, vulval cones and second-stage juveniles of the Xinjiang population were in the same range of each other and within those of other reported H. schachtii populations. Molecular analysis of rDNA-ITS, 28S-D2/D3 and mtDNA cytochrome c oxidase subunit 1 (COI) gene sequences suggested that the Xinjiang population clustered in a branch with those foreign populations, and the sequence similarity was as high as 99.81–100%. Moreover, this result was confirmed by PCR assay with species-specific primer SHF6 and rDNA2 of H. schachtii, and the pathogenicity test confirmed successful Xinjiang population reproduction in both plant hosts. In conclusion, based on morphological and molecular characterization, this study confirmed that the cyst nematode population collected from sugar beet fields in Xinjiang is H. schachtii. As far as we know, this is the first report of H. schachtii on sugar beets in Xinjiang, China.

Key words: sugar beet cyst nematode , Heterodera schachtii , morphological , molecular identification

. JIA-2021-0706 危害我国新疆甜菜的孢囊线虫种类首次鉴定[J]. Journal of Integrative Agriculture, 2022, 21(6): 1694-1702.

PENG Huan, LIU Hui, GAO Li, JIANG Ru, LI Guang-kuo, GAO Hai-feng, Wu Wei, WANG Jun, Zhang Yu, HUANG Wen-kun, KONG Ling-an, PENG De-liang. Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China[J]. Journal of Integrative Agriculture, 2022, 21(6): 1694-1702.

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JIA-2021-0706 危害我国新疆甜菜的孢囊线虫种类首次鉴定

Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China

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