Elimination of grapevine fleck virus and grapevine rupestris stem pitting-associated virus from Vitis vinifera 87-1 by ribavirin combined with thermotherapy

doi:10.1016/S2095-3119(20)63336-6

摘要/Abstract

摘要：

本研究以感染葡萄斑点病毒（grapevine fleck virus，GFkV）和沙地葡萄茎痘伴随病毒（grapevine rupestris stem pitting-associated virus，GRSPaV）的87-1葡萄试管苗作为试验材料，研究了不同浓度的病毒醚处理（15和25 μg·mL^-1; R15和R25）、热处理（37°C; T）以及病毒醚结合热处理（R15+T和R25+T）对葡萄试管苗中两种病毒的脱除效果。处理过程中发现，在R15和R25病毒醚处理的过程中，葡萄试管苗均产生了药害，主要表现为植株长势弱。热处理对葡萄植株的生长具有促进作用。T、R15+T和R25+T三个高温处理中植株的株高显著高于CK、R15和R25三个常温处理的植株株高。此外，两种方法的结合可以降低高温对植株的热损伤，处理结束时T、R15+T和R25+T三个处理的植株死亡率分别为51.4%、11.4%和8.6%。处理结束后切取一定大小的茎尖进行再生，各处理再生植株的存活率均高于68.0%。同时发现，病毒醚的处理时间与再生茎尖的成活率和两种病毒的脱除效率有关。R15+T处理30、40和50 d，植株存活率分别为97.3%、90.7%和74.4%。3个时间段中GRSPaV的脱除率分别为55.6%、84.6%和93.8%。此外，病毒醚的浓度也与两种病毒的脱除效率有关。R25(35/44)处理对GFkV的脱除率比R15(25/45)处理高23.9%，对GRSPaV的脱除率比R15处理高7.0%。对各处理的脱除效率进行分析发现，热处理结合化学处理可以明显促进GFkV和GRSPa的脱除，R25+T处理50天可完全脱除葡萄试管苗中的这两种病毒。

Abstract:

Vitis vinifera 87-1 plants infected by grapevine fleck virus (GFkV) and grapevine rupestris stem pitting-associated virus (GRSPaV) were used as the plant materials for virus elimination treatment. This study evaluated the effects of ribavirin at different concentrations (15 and 25 μg mL^–1; R15 and R25, respectively), thermotherapy (37°C; T), and the combination of ribavirin and thermotherapy (R15+T and R25+T) on eliminating viruses from grapevine plants in vitro. Both R15 and R25 had phytotoxic effects and weakened plant growth. Thermotherapy positively affected the growth of grapevine plants. Plant height was significantly greater in T, R15+T, and R25+T than in CK, R15 and R25. The proportion of dead plants after T, R15+T, and R25+T was 51.4, 11.4, and 8.6%, respectively. The survival rates of regenerated plants after all treatments were >68.0%. Ribavirin concentration and treatment time were related to the regeneration of shoot tips and elimination efficiencies of the two viruses. The survival rates of plants after R15+T for 30, 40, and 50 days were 97.3, 90.7, and 74.4%, respectively. The elimination rates of GRSPaV from plants in the three time quantum were 55.6, 84.6, and 93.8%, respectively. The elimination rate of GFkV was 23.9% higher in R25 (35/44) than in R15 (25/45), and that of GRSPaV was 7.0% higher in R25 than in R15. The combination of thermotherapy and chemotherapy was found to have a positive effect on the eradication of GFkV and GRSPaV, and R25+T for 50 days was able to completely eliminate the two viruses from in vitro grapevines.

Key words: in vitro grapevine , grapevine fleck virus (GFkV) , grapevine rupestris stem pitting-associated virus (GRSPaV) , chemotherapy , thermotherapy , virus elimination

. [J]. Journal of Integrative Agriculture, 2021, 20(9): 2463-2470.

HU Guo-jun, DONG Ya-feng, ZHANG Zun-ping, FAN Xu-dong, REN Fan. Elimination of grapevine fleck virus and grapevine rupestris stem pitting-associated virus from Vitis vinifera 87-1 by ribavirin combined with thermotherapy[J]. Journal of Integrative Agriculture, 2021, 20(9): 2463-2470.

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