Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (6): 1772-1785.DOI: 10.1016/S2095-3119(21)63752-8

所属专题： 农业生态环境-有机碳与农业废弃物还田合辑Agro-ecosystem & Environment—SOC

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JIA-2021-0336 秸秆和养分综合管理通过调控微生物分类单元和胞外酶来促进土壤新碳生成

  

  • 收稿日期:2021-02-23 接受日期:2021-05-24 出版日期:2022-06-01 发布日期:2021-05-24

Integrated management of crop residue and nutrients enhances new carbon formation by regulating microbial taxa and enzymes

WU Hong-liang1, CAI An-dong2, XING Ting-ting1, HUAI Sheng-chang1, ZHU Ping3, HAN Xiao-zeng4, XU Ming-gang5, LU Chang-ai1   

  1. 1 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land, Beijing 100081, P.R.China
    2 Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
    3 Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun 130033, P.R.China
    4 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R.China
    5 College of Resources and Environment, Shanxi Agricultural University, Taiyuan 030031, P.R.China
  • Received:2021-02-23 Accepted:2021-05-24 Online:2022-06-01 Published:2021-05-24
  • About author:WU Hong-liang, E-mail: wuhongliang@163.com; Correspondence LU Chang-ai, Tel/Fax: +86-10-82108703, E-mail: luchangai@caas.cn
  • Supported by:
    This work was financially supported by the Agro-scientific Research in the Public Interest of China (201503122), the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-XTCX2016008), and the National Natural Science Foundation of China (41620104006).

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摘要：

本研究通过84天的室内培养试验来揭示秸秆和养分（氮(N)、磷(P)和硫(S)）联合供应下土壤新碳生成的潜在微生物机制。结果表明，与对照土壤相比，单独添加秸秆刺激微生物进行养分开采，这与C:N和C:P酶活的比例降低了8-16%相吻合。随着养分补充水平的增提高，公主岭土壤新碳生成量从1155.9增加到1722.4 mg kg-1，海伦土壤则从725.1增加到1067.5 mg kg-1。回归树分析表明β-葡萄糖苷酶（BG）、酸性磷酸酶（AP）、微生物量碳（MBC）和酸杆菌对公主岭新碳生成的相对影响分别为27.8、18.5、14.7和8.1%；对海伦新碳生成的相对影响分别为25.9、29.5、10.1和13.9%。路径分析表明酸杆菌通过调节BG、AP和MBC直接或间接对土壤新碳生成产生积极影响，其中MBC的获取更多受到AP的调节。海伦土壤的新碳生成强度低于公主岭土壤，并且海伦土壤的新碳生成与AP活性直接相关，表明土壤属性（例如SOC和pH值）对土壤新碳生成的重要性。综上，本研究揭示了添加秸秆的土壤中新碳生成与NPS养分补充的响应关系，且土壤新碳生成主要依赖于酸杆菌和变形菌的生长代谢及对BG和AP的调控。


Abstract: Although returning crop residue to fields is a recommended measure for improving soil carbon (C) stocks in agroecosystems, the response of newly formed soil C (NFC) to the integrated supply of residue and nutrients and the microbial mechanisms have not been fully understood. Therefore, an 84-day incubation experiment was conducted to ascertain the microbial mechanisms that underpin the NFC response to inputs of residue and nitrogen (N), phosphorus (P), and sulfur (S) in two black soils. The results showed that adding residue alone accelerated microbial nutrient mining, which was supported by decreases of 8–16% in the ratios of C:N and C:P enzyme activities (relative to soils with nutrient inputs). The NFC amounts increased from 1155.9 to 1722.4 mg kg−1 soil in Gongzhuling and increased from 725.1 to 1067.5 mg kg−1 soil in Hailun as the levels of nutrient supplementation increased. Boosted regression tree analysis suggested that β-glucosidase (BG), acid phosphatase (AP), microbial biomass C (MBC), and Acidobacteria accounted for 27.8, 18.5, 14.7, and 8.1%, respectively, of the NFC in Gongzhuling and accounted for 25.9, 29.5, 10.1, and 13.9%, respectively, of the NFC in Hailun. Path analysis determined that Acidobacteria positively influenced NFC both directly and indirectly by regulating BG, AP, and MBC, in which MBC acquisition was regulated more by AP. The intensity of NFC was lower in Hailun soil than in Gongzhuling soil and was directly affected by AP, thereby indicating the importance of soil status (e.g., SOC and pH) in determining NFC. Overall, our results reveal the response of NFC to supplementation by N, P, and S, which depends on Acidobacteria and Proteobacteria, and their investment in BG and AP in residue-amended soil.

Key words: newly formed soil carbon ,  extracellular enzyme activities ,  gene abundance ,  nutrient supplementation ,  black soil