Genome-wide identification and expression analysis of Argonaute gene family from longan embryogenic callus

doi:10.1016/S2095-3119(20)63313-5

摘要/Abstract

摘要：

Argonaute（AGO）蛋白是RNA诱导基因沉默复合体的核心元件，调控植物从器官发育到非生物胁迫的各个过程。AGO基因家族在许多植物中已被鉴定，但在龙眼中并未见报道。此外，AGO如何在植物胚胎的信号通路中对变化的环境刺激作出反应也尚不清楚。本研究对龙眼的AGO基因家族进行了全基因组鉴定，并分析了其在体细胞胚胎发生（SE）、合子胚胎发生（ZE）、组织发育过程、激素反应、光照和非生物胁迫处理的表达模式。结果表明，基于龙眼基因组数据库有10个龙眼AGO基因被鉴定出来，分为4个分支，分布在1、4、8、10、12、13、14和15号染色体上，内含子数目介于2-23个。表达谱分析表明DlAGOs调控龙眼早中期胚胎、种子、花和茎的发育过程。此外，外源激素、光照和非生物胁迫对DlAGOs的转录水平也表现出不同的表达模式。这些研究结果为进一步阐明龙眼EC中RNAi介导的基因沉默机制提供了有用的信息。

Abstract:

Argonaute (AGO) proteins are the core of the RNA-induced gene silencing complex which regulate a wide variety of processes in plants, from organ development to abiotic stress responses. They have been identified in many plants, but little is known in longan (Dimocarpus longan Lour.), and how AGO functions in the signaling pathways in plant embryos in response to changing environmental stimuli remains unclear. In the present research, a genome-wide analysis of the AGO gene family members and their roles in somatic embryogenesis (SE), zygotic embryogenesis (ZE), tissue developmental processes, and responses to hormones, light and abiotic stress in longan were conducted. Ten longan AGO genes were identified genome-wide and divided into four clades. They were distributed on chromosomes 1, 4, 8, 10, 12, 13, 14, and 15, and had 2–23 introns. The expression profiling implied that DlAGOs regulated early and middle embryogenesis, as well as developmental processes of seed, flower, and stem in longan. In addition, the transcript levels of DlAGOs in response to exogenous hormones, light and abiotic stress showed differences in expression patterns. These results provide the useful information for further elucidation of RNAi-mediated gene silencing in longan embryogenic callus (EC).

Key words: Argonaute family , longan embryogenesis , expression profile , hormones , stress response

. [J]. Journal of Integrative Agriculture, 2021, 20(8): 2138-2155.

CHEN Rong-zhu, SHEN Xu, ZHANG Shu-ting, ZHAO Hua, CHEN Xiao-hui, XU Xiao-ping, HUO Wen, ZHANG Zi-hao, LIN Yu-ling, LAI Zhong-xiong. Genome-wide identification and expression analysis of Argonaute gene family from longan embryogenic callus[J]. Journal of Integrative Agriculture, 2021, 20(8): 2138-2155.

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