Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust, Locusta migratoria

doi:10.1016/S2095-3119(20)63248-8

摘要/Abstract

摘要：

表皮蛋白是昆虫表皮关联器官的主要组成部分，如体壁和翅，然而其在渐变态昆虫翅发育中的功能尚不清楚。本文从飞蝗中鉴定出一种属于CPR 家族RR-2亚家族的翅表皮蛋白LmACP8。LmACP8主要在翅芽中表达，且在三龄、四龄和五龄若虫蜕皮前表达量较高，其编码的蛋白定位于翅芽和成虫翅的原表皮层。利用RNA干扰，LmACP8的表达缺失显著降低了其蛋白质含量，从而导致飞蝗若虫向成虫转变过程中翅形态发生异常。进一步研究证实这种异常形态发生是由于翅内皮层严重受损所致。个体实验结果发现，蜕皮激素（20-hydroxyecdysone，20E）能够抑制LmACP8的表达，而在干扰激素受体基因LmHR39（Hormone receptor 39）后，LmACP8表达显著上调。由此可见，LmACP8参与飞蝗若虫向成虫转变过程中翅的发育，且其表达受LmHR39介导的20E信号通路负调控。

Abstract:

Cuticular proteins (CPs) are major components of the insect cuticle-associated organs such as integument and wings, although the importance of CPs for wing development and function in hemimetabolous insects remains understudied. In the present study, a wing cuticular protein LmACP8 was identified from Locusta migratoria, which belongs to the RR-2 subfamily of cuticular protein R&R consensus (CPR) chitin-binding proteins. LmACP8 was mainly expressed in the wing pads and showed high expression levels before ecdysis of third-, fourth-, and fifth-instar nymphs, with its encoded protein located in the procuticle of wing pads and adult wings. Depletion of LmACP8 by RNA interference markedly reduced the amount of its protein, which consequently caused abnormal wing morphogenesis in the transition from nymph to adult of L. migratoria. We further demonstrated that the abnormal morphogenesis was caused by severe damage of the endocuticle in the wings. LmACP8 was suppressed by 20-hydroxyecdysone (20E) in vivo, however, its expression was significantly up-regulated after knocking down the hormone receptor gene LmHR39. Thus, the LmACP8 that is negatively regulated by the LmHR39-mediated 20E signaling pathway is involved in wing development during the nymph to adult transition.

Key words: cuticular proteins , LmACP8 , 20-hydroxyecdysone , wing development

. [J]. Journal of Integrative Agriculture, 2021, 20(6): 1596-1606.

ZHAO Xiao-ming, YANG Jia-peng, GOU Xin, LIU Wei-min, ZHANG Jian-zhen. Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust, Locusta migratoria[J]. Journal of Integrative Agriculture, 2021, 20(6): 1596-1606.

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