Sugarcane mosaic virus infection of model plants Brachypodium distachyon and Nicotiana benthamiana

doi:10.1016/S2095-3119(19)62572-4

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (10): 2294-2301.DOI: 10.1016/S2095-3119(19)62572-4

收稿日期:2018-09-25 出版日期:2019-10-01 发布日期:2019-09-29

Sugarcane mosaic virus infection of model plants Brachypodium distachyon and Nicotiana benthamiana

XU Jing-sheng^{1, 2*}, DENG Yu-qing^{1, 2*}, CHENG Guang-yuan^{1, 2}, ZHAI Yu-shan^{1, 2}, PENG Lei^{1, 2}, DONG Meng^{1, 2}, XU Qian^{1, 2}, YANG Yong-qing^{2, 3}

¹ Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian) of Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
² Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
³ Root Biology Center, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China

Received:2018-09-25 Online:2019-10-01 Published:2019-09-29
Contact: Correspondence XU Jing-sheng, Tel/Fax: +86-591-83851472, E-mail: xujingsheng@126.com
About author: DENG Yu-qing, E-mail: dyqdyq999@163.com; * These authors contributed equally to this study.
Supported by:
Financial support was provided by the National Natural Science Foundation of China (31371688).

摘要/Abstract

Abstract:

Sugarcane mosaic virus (SCMV; genus Potyvirus, family Potyviridae) is a causal pathogen of sugarcane mosaic disease, and it is widespread in regions where sugarcane (Saccharum spp. hybrids) is grown. It is difficult to investigate the molecular mechanism of pathogen infection in sugarcane because of limited genomic information. Here, we demonstrated that SCMV strain FZ1 can systemically infect Brachypodium distachyon inbred line Bd21 and Nicotiana benthamiana through inoculation, double antibody sandwich enzyme-linked immunosorbent, transmission electron microscopy, and reverse transcription PCR assays. The leaves of Bd21 developed mosaic symptoms, while the leaves of N. benthamiana showed no obvious symptoms under the challenge of SCMV-FZ1. We concluded that B. distachyon inbred line Bd21 is a promising experimental model plant compared with N. benthamiana for study on the infectivity of SCMV. This is the first report on the SCMV infection of model plants B. distachyon inbred line Bd21 and N. benthamiana, which will shed light on the mechanism of SCMV infection of sugarcane and benefit sugarcane breeding against sugarcane mosaic disease.

Key words: SCMV , infection , sugarcane , Brachypodium distachyon , Nicotiana benthamiana

. [J]. Journal of Integrative Agriculture, 2019, 18(10): 2294-2301.

XU Jing-sheng, DENG Yu-qing, CHENG Guang-yuan, ZHAI Yu-shan, PENG Lei, DONG Meng, XU Qian, YANG Yong-qing. Sugarcane mosaic virus infection of model plants Brachypodium distachyon and Nicotiana benthamiana[J]. Journal of Integrative Agriculture, 2019, 18(10): 2294-2301.

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Sugarcane mosaic virus infection of model plants Brachypodium distachyon and Nicotiana benthamiana

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