JIA-2020-2539 小菜蛾羧酸酯酶基因PxαE8的鉴定及其在多药抗性中的作用

doi:10.1016/S2095-3119(21)63636-5

摘要/Abstract

摘要：

本文鉴定了一个小菜蛾的羧酸酯酶基因PxαE8，其开放阅读框共1599个核苷酸，编码532年氨基酸残基。对其时空表达谱研究表明，该基因在小菜蛾的不同发育阶段和不同部位或组织均有表达，尤其在幼虫、成虫以及幼虫的脂肪体、马氏管和血淋巴中高表达。进一步研究发现PxαE8在海南（HN）和广东（GD）两个多药抗性种群中的相对表达量分别是敏感种群的24.4-和15.5倍。利用RNAi干扰PxαE8在HN种群中的表达后再用LC₅₀的不同杀虫剂处理，发现用高效氯氰菊酯和辛硫磷处理可使其死亡率分别显著增加25.3%和18.3%。上述结果表明PxαE8的上调表达参与了小菜蛾对高效氯氰菊酯和辛硫磷的抗性，为进一步阐明小菜蛾和其他害虫对杀虫剂多药抗性的分子机制提供了基础。

Abstract:

Carboxylesterases (CarEs) were considered as important phase-I detoxifying enzymes which participated in detoxification of different types of insecticides. Up-regulation of CarE genes has been proved playing a major role in insecticide resistance in many pest insects, but its involvement in resistance to insecticides in Plutella xylostella has been rarely reported. In this study, a CarE cDNA named PxαE8 was identified in P. xylostella, which has an open reading frame of 1599 nucleotides and putatively encodes 532 amino acids. The investigation of spatial and temporal expression profiles of PxαE8 revealed that it was expressed in all developmental stages and body part/tissues, especially in larvae, adults as well as fat body, malpighian tubule and hemolymph of larvae. Further, the relative expression of PxαE8 in two multi-resistant field populations, Hainan (HN) and Guangdong (GD) populations, was found 24.4- and 15.5-fold higher than that in susceptible population, respectively. Knockdown of PxαE8 by RNAi dramatically increased the mortalities of larvae of HN population treated with LC₅₀of beta-cypermethrin and phoxim by 25.3 and 18.3%, respectively. These results suggested that up-regulation of PxαE8 was involved in resistance to both beta-cypermethrin and phoxim in P. xylostella, which shed light on further understanding of molecular mechanisms of multi-insecticide-resistance in P. xylostella and other pest insects.

Key words: Plutella xylostella , carboxylesterases , insecticide resistance , beta-cypermethrin , phoxim

. JIA-2020-2539 小菜蛾羧酸酯酶基因PxαE8的鉴定及其在多药抗性中的作用[J]. Journal of Integrative Agriculture, 2022, 21(6): 1713-1721.

LI Ran, SUN Xi, LIANG Pei, GAO Xi-wu. Characterization of carboxylesterase PxαE8 and its role in multi-insecticide resistance in Plutella xylostella (L.)[J]. Journal of Integrative Agriculture, 2022, 21(6): 1713-1721.

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