MdSWEET17, a sugar transporter in apple, enhances drought tolerance in tomato

doi:10.1016/S2095-3119(19)62695-X

Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (9): 2041-2051.DOI: 10.1016/S2095-3119(19)62695-X

收稿日期:2018-09-05 出版日期:2019-09-01 发布日期:2019-08-31

MdSWEET17, a sugar transporter in apple, enhances drought tolerance in tomato

LU Jing^1*, Sun Mei-hong^2*, MA Qi-jun¹, KANG Hui¹, LIU Ya-jing¹, HAO Yu-jin¹, YOU Chun-xiang¹

¹ National Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
² Institute of Plant Biotechnology, College of Life Sciences, Shanghai Normal University, Shanghai 200234, P.R.China

Received:2018-09-05 Online:2019-09-01 Published:2019-08-31
Contact: Correspondence YOU Chun-xiang, E-mail: youchunxiang@126.com; HAO Yu-jin, E-mail: haoyujin@sdau.edu.cn
About author:LU Jing, E-mail: 609636775@qq.com; * These authors contributed equally to this work.
Supported by:
This work was supported by the National Natural Science Foundation of China (31471854, 31772288) and the Natural Science Foundation of Shandong Province, China (ZR2017ZC0328).

摘要/Abstract

Abstract:

Sugars are important energy source and signaling molecule in plant, and sugar transporters such as SWEET (sugars will eventually be exported transporters) play important roles in plant growth and development as well as biotic and abiotic stresses. In this study, a gene named MdSWEET17 was isolated from apple (Malus×domestic). Expression analysis suggested that MdSWEET17 expressed in all tested tissues (root, stem, leaf, flower and fruit) and the transcript levels were different. Furthermore, MdSWEET17 transgenic tomatoes and wild type were treated with drought. The results showed transgenic lines had higher drought tolerance and accumulated more fructose. Taken together, these findings indicated that MdSWEET17 take part in drought stress response and the regulation of fructose.

Key words: apple , MdSWEET17 , drought , soluble sugar , tomato

. [J]. Journal of Integrative Agriculture, 2019, 18(9): 2041-2051.

LU Jing, Sun Mei-hong, MA Qi-jun, KANG Hui, LIU Ya-jing, HAO Yu-jin, YOU Chun-xiang . MdSWEET17, a sugar transporter in apple, enhances drought tolerance in tomato[J]. Journal of Integrative Agriculture, 2019, 18(9): 2041-2051.

参考文献

Babita M, Maheswari M, Rao L M, Shanker A K, Rao D G. 2010. Osmotic adjustment, drought tolerance and yield in castor (Ricinus communis L.) hybrids. Environmental and Experimental Botany, 69, 243–249.
Baker R F, Leach K A, Braun D M. 2012. SWEET as sugar: New sucrose effluxers in plants. Molecular Plant, 5, 766–768.
Chardon F, Bedu M, Calenge F, Klemens P A W, Spinner L, Clement G, Chietera G, Léran S, Ferrand M. 2013. Leaf fructose content is controlled by the vacuolar transporter SWEET17 in Arabidopsis. Current Biology, 23, 697–702.
Chen H, Jung H, Yu Y, Li H, Chen L, Dorothea T. 2015. The Arabidopsis vacuolar sugar transporter sweet2 limits carbon sequestration from roots and restricts pythium infection. Journal of Molecular Cell Biology, 83, 1046–1058.
Chen L Q, Hou B H, Lalonde S. 2010. Sugar transporters for intercellular exchange and nutrition of pathogens. Nature, 468, 527–532.
Chen L Q, Lin I W, Qu X Q, Sosso D, Mcfarlane H E, Londoño A, Samuels A L, Frommer W B. 2015. A cascade of sequentially expressed sucrose transporters in the seed coat and endosperm provides nutrition for the Arabidopsis embryo. The Plant Cell, 27, 607.
Durand M, Mainson D, Porcheron B, Maurousset L, Lemoine R, Pourtau N. 2018. Carbon source-sink relationship in Arabidopsis thaliana: The role of sucrose transporters. Planta, 247, 587–611.
Engel M L, Holmes D R, Cormick S. 2005. Green sperm. Identification of male gamete promoters in Arabidopsis. Plant Physiology, 138, 2124–2133.
Fu J M, Huang B R. 2001. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environment and Experimental Botany, 45, 105–114.
Finkelstein R R, Gibson S I. 2001. ABA and sugar interactions regulating development: cross-talk or voices in a crowd? Current Opinion in Plant Biology, 5, 26–32.
Gill P K, Sharma A D, Singh P, Bhullar S S. 2003. Changes in germination, growth and soluble sugar contents of Sorghum bicolor (L.) seeds under various abiotic stresses. Plant Growth Regulation, 40, 157–162.
Gorham J, Hughes L Y, Wynjones R G. 1981. Low molecular weight carbohydrates in some salt stressed plants. Physiologia Plantarum, 53, 27–33.
Guan Y F, Huang X Y, Zhu J, Gao J F, Zhang H X, Zhong N, Yang Z N. 2008. RUPTURED POLLEN GRAIN1, a member of the MtN3/saliva gene family, is crucial for exine pattern formation and cell integrity of microspores in Arabidopsis. Plant Physiology, 147, 852–863.
Guo W J, Nagy R, Chen H Y, Pfrunder S, Yu Y C, Santelia D, Frommer W B, Martinoia E. 2014. SWEET17, a facilitative transporter, mediates fructose transport across the tonoplast of Arabidopsis roots and leaves. Plant Physiology, 164, 777–789.
Hir R L, Spinner L, Klemens P A W, Chakraborti D, Marco F D, Vilaine F O, Wolff N, Lemoine R, Porcheron B, Géry C, Téoulé E, Chabout S, Mouille G, Neuhaus H E, Dinant S, Bellini C. 2015. Disruption of the sugar transporters AtSWEET11 and AtSWEET12 affects vascular development and freezing tolerance in Arabidopsis. Molecular Plant, 8, 1687–1690.
Hossain M A, Cho J I,Han M, Ahn C H, Jeon J S, An G, Park P B. 2010. The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice. Journal of Plant Physiology, 167, 1512–1520.
Hou P F, Ma J Q, Zhao P F, Zhang H L, Zhao H J, Liu H S, Zhao Y D, Wang Y X. 2013. Effects of betaine on chloroplast protective enzymes and psbA gene expression in wheat seedlings under drought stress. Acta Agronomica Sinica, 39, 1319. (in Chinese)
Kanno Y, Oikawa T, Chiba Y, Ishimaru Y, Shimizu T, Sano N, Koshiba T, Kamiya Y, Ueda M, Ueda M. 2016. AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes. Nature Communacations, 7, 13245.
Kidokoro S, Watanabe K, Ohori T, Moriwaki T, Maruyama K, Mizoi J, Myint P, Fujita Y, Sekita S, Shinozaki K. 2015. Soybean DREB1/CBF-type transcription factors function in heat and drought as well as cold stress-responsive gene expression. The Plant Journal, 81, 505–518.
Klemens P A W, Patzke K, Deitmer J, Spinner L, Hir R L, Catherine B C, Bedu M, Chardon F, Krapp A, Neuhaus H E. 2013. Overexpression of the vacuolar sugar carrier AtSWEET16 modifies germination, growth, and stress tolerance in Arabidopsis. Plant Physiology, 163, 1338–1352.
Lei M. 2008. Influence of plant growth regulator, sugar and light quality on fruit quality of red globe grapes. Anhui Agricultural UniVersity. (in Chinese)
Ling Y J, Mo Q, Mo F, Ge C, Luo S, Luo Y. 2018. Effects of exogenous sugar treatment on fruit quality and main bioactive compounds in strawberry. Journal of Sichuan Agricultural University, 36, 67–71. (in Chinese)
Liu X Z, Zhang Y, Yang C, Tian Z H, Li J X. 2016. AtSWEET4, a hexose facilitator, mediates sugar transport to axial sinks and affects plant development. Scientific Reports, 6, 24563.
Ma Q J, Sun M H, Kang H, Lu J, You C X, Hao Y J. 2019. An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought. Plant Biotechnology Journal, 17, 625–637.
Ma Q J, Sun M H, Lu J, Zhu X P, Gao W S, Hao Y J. 2017. Ectopic expression of apple MdSUT2 gene influences development and abiotic stress resistance in tomato. Science Horticultrea, 220, 259–266.
Minorsky P V. 2003. Raffinose oligosaccharides. Plant Physiology, 131, 1159–1160.
Morsy M R, Jouve L, Hausman J F, Hoffmann L, Stewart J D. 2007. Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. Plant Physiology, 164, 157–167.
Mostajeran A, Rahimi E V. 2009. Effects of drought stress on growth and yield of rice (Oryza sativa L.) cultivars and accumulation of proline and soluble sugars in sheath and blades of their different ages leaves. American-Eurasian Journal Agricultural Environment Science, 5, 264–272.
Murakeozy E P, Nagy Z, Duhaze C, Bouchereau A, Tuba Z. 2003. Seasonal changes in the levels of compatible osmolytes in three halophytic species of inland saline vegetation in hungary. Plant Physiology, 160, 395–401.
Nayer M, Reza H. 2008. Drought-induced accumulation of soluble sugars and proline in two maize varieties. World Applied Sciences Journal, 3, 448–453.
Norwood M, Toldi O, Richter A, Scott P. 2003. Investigation into the ability of roots of the poikilohydric plant Craterostigma plantagenium to survive dehydration stress. Journal of Experimental Botany, 54, 2313–2321.
Pandey D M, Yeo U D. 2008. Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf. Biologia Plantarum, 52, 291–298.
Pilon-Smits E A H, Terry Norman, Sears T, van Dun K. 1999. Enhanced drought resistance in fructan-producing sugar beet. Plant Physiology and Biochemistry, 37, 313–317.
Ranganayakulu G S, Veeranagamallaiah G, Sudhakar C. 2013. Effect of salt stress on osmolyte accumulation in two groundnut cultivars (Arachis hypogaea L.) with contrasting salt tolerance. African Journal of Plant Science, 7, 586–592.
Rosa M, Hilal M, González J A, Prado F E. 2004. Changes in soluble carbohydrates and related enzymes induced by low temperature during early developmental stages of quinoa (Chenopodium quinoa) seedlings. Journal of Plant Physiology, 161, 683–689.
Seo P J, Park J M, Kang S K, Kim S G, Park C M. 2011. An Arabidopsis senescence-associated protein SAG29 regulates cell viability under high salinity. Planta, 233, 189–200.
Shao H B, Liang Z S, Shao M A. 2006. Osmotic regulation of 10 wheat (Triticum aestivum L.) genotypes at soil water deficits. Colloid and Surfaces (B: Biointerfaces), 47, 132–139.
Siringam K, Juntawong N, Chaum S, Kirdmanee C. 2011. Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under isoosmotic conditions. African Journal of Biotechnology, 10, 1340–1346.
Slewinski T L. 2011. Diverse functional roles of monosaccharide transporters and their homologs in vascular plants: A physiological perspective. Molecular Plant, 4, 641–662.
Smirnoff N. 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist, 125, 27–58.
Sun M X, Huang X Y, Yang J, Guan Y F, Yang Z N. 2013. Arabidopsis RPG1 is important for primexine deposition and functions redundantly with RPG2 for plant fertility at the late reproductive stage. Plant Reproduction, 26, 83–91.
Talbot N J. 2010. Raiding the sweet shop. Nature, 468, 527–532.
Wang H B, Chen X S, Xin P G, Zhang X Y, Ci Z J. 2007. Study on sugar and acid constituents in several early apple cultivars and evaluation of their flavor quality. Journal of Fruit Science, 24, 513–516. (in Chinese)
Wang Y Z, Zhang D P. 2000. Regulating effects of ethylene on carbohydrate metabolism in Starkrimson apple fruit during the ripening period. Acta Horticulturae Sinica, 27, 391–395. (in Chinese)
Yang B, Sugio A, White F F. 2006. Os8N3 is a host disease-susceptibility gene for bacterial blight of rice. Proceedlings of the National Academy of Sciences of the United States of America, 103, 10503–10508.
Yuan M, Chu Z, Li X, Xu C, Wang S. 2010. The bacterial pathogen Xanthomonas oryzae overcomes rice defenses by regulating host copper redistribution. The Plant Cell, 22, 3164–3176.
Zhou A, Ma H, Feng S, Gong S, Wang J. 2018a. A novel sugar transporter from Dianthus spiculifolius, DsSWEET12, affects sugar metabolism and confers osmotic and oxidative stress tolerance in Arabidopsis. International Journal of Molecular Sciences, 19, 497.
Zhou A, Ma H, Feng S, Gong S, Wang J. 2018b. DsSWEET17, a tonoplast-localized sugar transporter from Dianthus spiculifolius, affects sugar metabolism and confers multiple stress tolerance in Arabidopsis. International Journal of Molecular Sciences, 19, 1564.

MdSWEET17, a sugar transporter in apple, enhances drought tolerance in tomato

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