Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism

doi:10.1016/S2095-3119(17)61878-1

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (09): 1991-1998.DOI: 10.1016/S2095-3119(17)61878-1

收稿日期:2017-09-29 出版日期:2018-09-01 发布日期:2018-08-14

Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism

ZHANG Xiang^{1, 2}, RUI Qiu-zhi¹, LIANG Pan-pan¹, WEI Chen-hua¹, DENG Guo-qiang¹, CHEN Yuan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">CHEN Yuan¹, CHEN Yuan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">CHEN Yuan¹, DONG Zhao-di¹, CHEN De-hua¹#br#

¹ Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, P.R.China
² Plant Genome Mapping Laboratory, University of Georgia, Athens 30605, USA

Received:2017-09-29 Online:2018-09-01 Published:2018-08-14
Contact: Correspondence CHEN De-hua,Tel:+86-514-87979357,Fax:+86-514-87996817,E-mail:cdh@yzu.edu.cn
About author:ZHANG Xiang,Tel:+86-514-87979357,E-mail:yzzhangxiang@163.com;
Supported by:
This work was supported by the National Natural Science Foundation of China (31471435, 31671613, and 31301263), the China Postdoctoral Science Foundation Grant (2016M591934), the Postdoctoral Science Foundation Grant in Jiangsu Province, China (1601116C), the Key Projects of Natural Science Research in Colleges and Universities of Jiangsu, China (17KJA210003), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), and the Practice Innovation Training Project for College Students in Jiangsu Province, China.

摘要/Abstract

Abstract: This study was conducted to investigate the effects of alternating high temperature on Cry1Ac protein content on Bt cotton cultivars Sikang 1 (SK-1, a conventional cultivar) and Sikang 3 (SK-3, a hybrid cultivar). In 2011 and 2012, cotton plants were subjected to high temperature treatments ranging from 32 to 40°C in climate chambers to investigate the effects of high temperature on boll shell insecticidal protein expression. The experiments showed that significant decline of the boll shell insecticidal protein was detected at temperatures higher than 38°C after 24 h. Based on the results, the cotton plants were treated with the threshold temperature of 38°C from 6:00 a.m. to 6:00 p.m. followed by a normal temperature of 27°C during the remaining night hours (DH/NN) in 2012 and 2013. These treatments were conducted at peak boll growth stage for both cultivars in study periods of 0, 4, 7, and 10 d. Temperature treatment of 32°C from 6:00 a.m. to 6:00 p.m. and 27°C in the remaining hours was set as control. The results showed that, compared with the control, after the DH/NN stress treatment applied for 7 d, the boll shell Cry1Ac protein content level was significantly decreased by 19.1 and 17.5% for SK-1 and by 15.3 and 13.7% for SK-3 in 2012 and 2013, respectively. Further analysis of nitrogen metabolic physiology under DH/NN showed that the soluble protein content and the glutamic pyruvic transaminase (GPT) activities decreased slightly after 4 d, and then decreased sharply after 7 d. The free amino acid content and the protease content increased sharply after 7 d. The changes in SK-1 were greater than those in SK-3. These results suggest that under DH/NN stress, boll shell Cry1Ac protein content decline was delayed. Reduced protein synthesis and increased protein degradation in the boll shell decreased protein content, including Bt protein, which may reduce resistance to the cotton bollworm.

Key words: Bt cotton , alternating temperature , Cry1Ac protein , nitrogen metabolism

. [J]. Journal of Integrative Agriculture, 2018, 17(09): 1991-1998.

ZHANG Xiang, RUI Qiu-zhi, LIANG Pan-pan, WEI Chen-hua, DENG Guo-qiang, CHEN Yuan, CHEN Yuan, DONG Zhao-di, CHEN De-hua. Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism[J]. Journal of Integrative Agriculture, 2018, 17(09): 1991-1998.

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Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism

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